Tissue Culture Media We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a res ...
ORGANOTYPIC KIDNEY CULTURES -embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. -metanephroi and associated ureteric buds are microdissected and placed in holding medium (L ...
ORGANOTYPIC LIMB CULTURES -embryos are dissected from timed-pregnant mice from 10.5 - 11.5 d.p.c. -limb buds are microdissected and placed in holding medium (L15 medium supplemented with 1 x MITO+ ser ...
Isolation of Lung Bud Endoderm What you need: E11-12 mouse embryos DMEM with 5% fetal bovine serum petri dishes for dissections and washes Tyrode-Ringer's solution pH 7.6-7.7 (recipe below) pancreatin ...
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose no pyruvate) 7% FBS and 1X ...
In general terms cultured cells require a sterile environment and a supply of nutrients for growth. In addition the culture environment should be stable in terms of pH and temperature. Over the last ...
Cloning by Limiting Dilution of HybridomaAuthor: Nanci DonackiSource: Contributed by Nanci DonackiMaterials DMEM high glucose (Life Technologies Inc. #10313-021 or equivalent)Fetal Bovine Serum (Li ...
CULTURE MEDIUM Ingredients these catalog numbers refer to products from Sigma; products from other suppliers can also be used Powdered Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Feta ...
CULTURE MEDIUM Ingredients these catalog numbers refer to products from Sigma; products from other suppliers can also be used Powdered Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Feta ...
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept. University of Flori ...
Mixing F-12 (HAM) Media1) Start by rinsing a graduated cylinder three times ddH2O. 2) Then fill the cylinder to 600-ml with ddH2O. 3) Add one bottle of F-12 (HAMS) (Sigma #N-4388). 4) Stir this until ...
Culturing Chick Embryonic Cardiac Myocytes and FibroblastsMaterials1. Culture medium: F12K without glutamine 3% FCS 100 units/ml 100 ug/ml streptomycin (myocyte medium also for mixed culture); and F12 ...
Culturing Chick Embryonic Myoblasts/Myotubes and FibroblastsDay 1Materials1. 100 ug/ml gelatin (described separately later). Procedure1. Pipet 10 ml gelatin onto each of two 100 mm culture dishes. Lea ...
Human Microvascular Endothelial CellsCC-2505HMVEC-d NeoDermal - Neonatal500000 cells/ampCC-2516HMVEC-d NeoDermal - Neonatal(Pooled from500000 cells/ampseveral donors)CC-2543HMVEC-d AdDermal - Adu ...
Human Microvascular Endothelial CellsCC-2505HMVEC-d NeoDermal - Neonatal500000 cells/ampCC-2516HMVEC-d NeoDermal - Neonatal(Pooled from500000 cells/ampseveral donors)CC-2543HMVEC-d AdDermal - Adu ...
Lacrimal Experimental Protocol (4/4/94)Day Before: 1. Prepare (6) 50 ml conicals each containing 50 ml of DMEM (high glucose) and gentamycin (50 µg/ml); place in incubator (90% O2 10% CO2).2. Thaw FBS ...
MN Analysis : Blood Lymphocytes MN in Human Lymphocytes (method description)Lymphocyte isolationLymphocytes were isolated using Ficoll-Paque density gradients. Blood was diluted 1:1 with phosphat ...
MN Analysis : Blood Lymphocytes MN in Human Lymphocytes (method description)Lymphocyte isolationLymphocytes were isolated using Ficoll-Paque density gradients. Blood was diluted 1:1 with phosphat ...
ES Cell Culture and ManipulationCulturing ES cellsMedia High glucose DMEM (-pyruvate -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type be sure!!)1X l-glutamine1X Penicillin/stre ...
ES Cell Culture and ManipulationCulturing ES cellsMedia High glucose DMEM (-pyruvate -glutamine)20% Heat-inactivated Fetal calf serum (can vary by cell type be sure!!)1X l-glutamine1X Penicillin/stre ...
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