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        Cloning by Limiting Dilution of Hybridoma

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        Cloning by Limiting Dilution of Hybridoma

        Author: Nanci Donacki
        Source: Contributed by Nanci Donacki
         

         Materials
        1.   DMEM, high glucose (Life Technologies, Inc. #10313-021 or equivalent)
        2.   Fetal Bovine Serum (Life Technologies, Inc. #16000-044 or equivalent)
        3.   L-glutamine (Life Technologies, Inc. #25030-149 or equivalent)
        4.   Hybridoma Cloning Factor (Fisher # IG50-0615)
        5. 50 ml sterile centrifuge tubes ( Falcon #2070)
        6. 15 ml sterile centrifuge tubes (Falcon # 2099)
        7. 96 well culture plates (Falcon #3072))
        8.   Hemocytometer
        9. Trypan Blue, 0.4% (Life Technologies, Inc. # 630-5150AG)
        10. Multi-channel pipettor and sterile tips
        11. Reagent Reservoir
        12. HT (Life Technologies, Inc. #11067-030)

         

          Procedure

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