细胞技术

Differentiate ES cells into glial cells and neuronsDay -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1: Trypsin ...

Isolation of Primary Fibroblasts from Mouse Embryos 1. Treat 10 cm tissue culture plates with 0.1% gelatin for at least two hours before use. 2. Sacrifice the pregnant female mouse (day 13 or 14 p.c.) ...

Introduction The technique described here is a slight modification (March 1997) of methods presented in: Nagy A. J. Rossant. 1993. Production of completely ES cell-derived fetuses. InGene targeting ...

I. ProcedureHarvest cells from tissue preparing a single cell suspension. Red blood cells may be removed by lysis or density gradient: Red blood cells from murine peripheral blood or a spleen cell sus ...

Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry William Telford Hospital for Special Surgery This protocol is for use with the D and E cyclins and employs 488 ...

Conjugation of monoclonal antibodiesPrelude: You are free to copy and distribute these documents at will--but please do so in their entirety complete with figures. To reference this material please in ...

Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela J. Fraker Michian State University East Lansing M ...

Staining Procedure for Flow Cytometric Detection of Human CyclinsThis is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a goo ...

FACS Analysis Using Peripheral Blood CellsCollect blood (75 microliters) into 1ml PBS containing 5 microM EDTA (10 microliters of 0.5 M stock) and mix immediately to prevent clotting. Keep tubes on ic ...

Materials Monolayer cultures grown in 75 mm culture flasks (Cells from Exercise 11.4 may be used or cultures of tetrahymena yeast or algae may be used.)H-thymidine with at least 4 µc/ml 0.36 c/mMPhosp ...

Tissue Culture - Thawing Cells from Liquid NitrogenMETHOD:Prepare before thawing:Fill a test tube of 10 - 15 mls of cold media (appropriate for your cell line) with 10 - 20% fetal calf serum (use 2x t ...

A: Development of Immuno-LCM Limitation of Microdissection Microdissection of routinely stained or unstained frozen sections has been used successfully to obtain purified cell populations for the anal ...

This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research obj ...

Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction21.1.1Terminology of cell death 21.1.2Differences between necrosis and apoptosis31.1.3Apoptotic Pathways4 1.2 Apoptosis Assay Methods6 Me ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. WilcoxJosé C. Rodriguez and Héctor de Léon - Emory University - April 1996P ...

In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809) modified by Josiah N. Wilcox andJosé C. Rodriguez - Emory University - April 1996Protocol for Fro ...

death Z. Xia 10/2/95The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green alive cells; P.I. (propidium iodide)-red dead cells 35 mm plates: to 2 ml med ...

1) Aliquot 5 X 106 total cells in 5-8 ml 2% media.2) Add 30 µl 3H-thymidine.3) Incubate for approximately 16 hours under normal growth conditions.4) Spin down cells and wash 2-3 times with PBS (or ser ...

Chemical Induction of Apoptosis - 1 May 2001 p53 p21WAF1 Myc Bcl-2 Bax Bcl-x and bak are among the proteins involved in the regulation of apoptosis. The agents and doses listed in the table below have ...

Transient transfection into 293T cells (by Jun Takagi Jun 152000)PurposeTransient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted o ...