细胞技术

cell proliferation assaybefore start:thaw cells from liquid nitrogen grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until content gets cloudy and starts to turn yellow (usually w ...

IntroductionQuality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of the cultures and products derived from ...

Preparation of low density collagenase-digested splenocytes Protocol 1. Dilute 2 ml of 4000 U/ml Collagenase D as follows: 1 ml into 9 ml HBSS/Ca++/Mg++ (=400U/ml) and 1 ml into 39 ml HBSS/Ca++/Mg++ ...

Defrosting Eukaryotic Cells Frozen In Liquid N2 Reagents / Solutions Frozen cell line. Growth media (e.g. RPMI1640) Foetal calf serum (FCS). ProtocolMake 20ml growth media/10% FCS for each vial of ce ...

Eukaryote Growth DynamicsLEVEL II Materials Suspension cultures set up from Exercise 12.8Sterile transfer pipettesMaterials for viability counting (Exercise 12.7) Procedure After 12 hours aseptically ...

Use of the Hemacytometer for the Determination of Cell Numbers Counting cells by the use of a hemacytometer is a convenient and practical method of determining cell numbers in the case that the Coulte ...

Viability Cell CountMaterials Suspension culture of cellsSterile transfer pipettesStock 0.2% (w/v) Trypan blueHemacytometer and microscope Procedure Gently swirl a suspension culture to distribute the ...

Load both sides of the hemacytometer with the cell suspension - covering both counting grids. Count the number of cells in 5 out of 9 squares from each counting grid (count a total of 10 squares). Div ...

Recommended working medium trypsin volume and cell inoculation densityAuthor: Long-Cheng LiSource: Abstract: Useful data for cell subculture with different culture vessels. ...

Transfer of Eukaryote Suspension CulturesLEVEL II Materials Fibroblast suspension cultureTissue culture laminar flow hoodMedia appropriate to culture line usedDisposable pipettes (10 ml and 1.0 ml)Dis ...

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept. University of Flori ...

This workbook was developed for use with Module 2 of the InVitro Insights Cell Culture Training Program developed by Becton Dickinson. The problem that follow are presented in increasing degress of co ...

Infection with retroviruses It is well established with avian retroviruses that cells are most efficiently infected just after they are trypsinized. Trypsinization does two things. First it apparently ...

Establishment of a Primary CultureLEVEL III Materials Chick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors forceps and probesSterile petri platesPhosphate buffered sal ...

Staining protocols for placental explant culturesWhole mount staining.The protocol given uses a single primary antibody with nuclear counterstaining but it can be extended to double antibody staining. ...

From early post-implantation stages to approximately 18 weeks' gestation cytotrophoblast at the tips of anchoring villi at the placental periphery proliferate to form columns. From the distal edges of ...

Components and Supplementation of Various Culture MediaAbbreviations: DMEM = Dulbecco's Modified Eagle's Medium FCS = Fetal Calf (or Bovine) Serum GPS = Glutamine-Penicillin-Streptomycin BME = b-Merca ...

TISSUE CULTURE STOCK SOLUTIONS AND MEDIAMS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:Sucrose ........... 10.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autocl ...

Tissue Culture Media We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a res ...

FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose no pyruvate) 7% FBS and 1X ...