细胞技术

Prostate gland development, growth, function and pathology are strongly influenced by steroid hormones. Androgens greatly influence the prostate, but the physiological maintenance of the gland can also depend on the presence of estrogens and other steroid hormones, retinoids and ...

Developmental processes are intricate networks of events by which a fertilized egg proliferates and differentiates into a fully functional multicellular organism (1). Mechanisms are used by dividing cells to create asymmetries in their information content that then allow the eme ...

In vertebrate animals, adipose tissue functions primarily to store excess energy in the form of triglycerides in periods of nutritional abundance and mobilize it in response to fasting (1,2). It also plays important roles in fatty acid metabolism and glucose homeostasis (3). Recently, adi ...

We have applied the differential display (DD) technique (1) to isolate genes whose expression is regulated in the brain of songbirds when they hear song of their own species. Song is known to cause a marked increase in mRNA levels of two immediate early genes, ZENK and c-jun, in the auditory forebrain of son ...

Differential display is ideally suited for the identification of genes involved in complex physiological events. Unlike subtractive hybridization techniques, differential display requires no previous knowledge about the dynamics of “important” gene products. Since the o ...

In order to develop angiogenic and antiangiogenic strategies, concerted efforts have been made to provide animal models for more quantitative analysis of in vivo angiogenesis. In vivo techniques consist of the cornea pocket and iris implant in the eye, the rabbit ear chamber, the dorsal skin ...

The idea of antiangiogenesis as a therapeutic strategy has been around for several decades (1). Vigorously pursued as a novel anticancer strategy (reviewed in (2–6), it is now widely considered to be a promising approach to the treatment of a range of pathologies of which uncontrolled vascular p ...

Although it has been recognized for many centuries that neoplastic tissue is more vascular than its normal counterpart, it is only since Folkmans’ hypothesis on antiangiogenesis (1) that a more quantitative method for measuring angiogenesis in tissue sections has been pursued. Folkman ...

Angiogenesis, the process of new blood vessels forming from preexisting vessels, is an important feature in developmental processes, wound healing, and pathologic conditions such as cancer and vascular diseases. Owing to the importance of angiogenesis, a relatively simple and rapid ...

Recently, the acceleration (and retardation) of blood vessel growth has been an increasingly frequent subject of study. With its potential application to a wide range of clinical disease processes, investigation certainly remains essential and promising. While in vitro investig ...

The aim of our research was to develop a quantitative assay for angiogenesis in mammals, especially the mouse. This is a convenient experimental animal because of its small size, which allows compact housing and experimentation with angiogenic factors or inhibitors in limited supply. Mou ...

The process of capillary growth, angiogenesis, is an integral part of wound healing and repair mechanisms. When it occurs during these conditions, it is tightly controlled and strictly delimited. However, in tumor growth and in a variety of vascular diseases, unrestrained angiogenesis c ...

Since 1924, when the first transparent chamber model in animals was introduced by Sandison (1), many other chamber models have been described in the literature for studying angiogenesis and microcirculation in a wide variety of neoplastic and nonneoplastic tissues by means of intravit ...

It is becoming increasingly clear that the process of angiogenesis is important for tumor growth and metastasis and as such provides an exploitable target for therapeutic intervention. Consequently a number of useful model systems has been developed to investigate the angiogenic pr ...

The study of the angiogenic process and the search for novel therapeutic agents to inhibit, or stimulate, angiogenesis has employed a wide range of in vivo ’angiogenesis’ assays (reviewed in 1–3). These differ greatly in their difficulty, quantitative nature, rapidity, and cost. The classic ...

The inner lining of blood vessels, the endothelium, consists of a monolayer of endothelial cells (ECs), that present a free luminal surface and attach on their abluminal side to the underlying basement membrane (apart from a minimal amount of cell-cell overlap). A great deal of heterogeneity ex ...

Angiogenesis is a complex morphogenetic process involving the coordinate migration of several cell types, including endothelial cells (EC), pericytes, and stromal fibroblasts (1–4). Angiogenesis is regulated by interactions between cells, soluble factors, and extracellu ...

Angiogenesis is a necessary component of normal tissue repair, tumor growth and dissemination, and a wide variety of other inflammatory and pathological processes as well, including diabetic retinopathy, rheumatoid arthritis, and psoriasis. Consequently, the last two decades h ...

A variety of in vivo and in vitro methods have been used to study angiogenesis, the process of blood vessel formation. Two widely accepted but technically difficult assays include the cornea implant assay and the chick chorioallantoic membrane assay. The cornea assay requires special equi ...

Human endothelial cells (EC) can be readily prepared from large vessels such as the aorta, and umbilical (1) and saphenous veins. ECs derived from these vessels have proven an abundant, convenient, and useful tool for the investigation of many aspects of endothelial biology. However, the major ...