Identification of Vertebrate Circadian Clock-Regulated Genes by Differential Display
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Differential display is ideally suited for the identification of genes involved in complex physiological events. Unlike subtractive hybridization techniques, differential display requires no previous knowledge about the dynamics of “important” gene products. Since the only limitation is the number of samples that can be run on a single sequencing gel, comparison of many subtly different conditions can be assessed, and mRNAs that vary in interesting ways can be identified.
