细胞技术

Baculoviruses have been used over the last several decades for high-level protein production in insect cells. Recently, modified baculovirus containing a mammalian promoter, known as BacMam virus, has been shown to give high transduction efficiencies across several cell types with ...

Small RNA suppression constitutes one of the major difficulties for a full molecular characterization of their specific roles in plants. Taking advantage of the latest insights into the new post-biogenesis layer of regulation in microRNA (miRNA) activity, it is possible to overcome the a ...

MicroRNAs (miRNAs) are a class of small noncoding transcripts that repress gene expression by pairing with their target messenger RNAs (mRNAs). The human genome codes for hundreds of different miRNAs and it is predicted that they target thousands of mRNAs involved in a wide variety of physiol ...

MicroRNAs (miRNAs) represent a class of small noncoding RNAs that negatively regulate gene expression�. Intensive research during the past decade has established miRNAs as key regulators of many cellular pathways. MiRNAs have also been implicated in a number of diseases including var ...

How eukaryotic organisms regulate mRNA levels is a fundamental question in biology. It is clear that the steady-state concentration of RNA in a cell is determined by both the rate of its synthesis and turnover. Most of the early attention was focused on the study of gene transcription, while only rece ...

microRNAs (miRNAs) regulate gene expression through sequence-specific interactions with cognate mRNAs that result in translational inhibition, mRNA decay, or slicing within the region of complementarity. miRNA processing activity on complementary target mRNAs genera ...

MicroRNAs (miRNAs) are small RNA molecules that posttranscriptionally regulate the expression of protein-coding genes. The mature miRNAs are loaded into Argonaute-containing protein complexes (miRISC, miRNA Induced S  ilencing Complex), and guide these complexes to the 3′ UTR of t ...

Small RNA (sRNA)-mediated gene silencing constitutes a powerful tool for the molecular characterization� of a given gene. The RNAi technology has been largely used for this purpose. This approach is based on the cloning of an inverted repeated fragment of the gene to be silenced. Even when this app ...

MicroRNAs (miRNAs) are noncoding RNA molecules that have come to attract considerable interest for their roles in animal and plant development and disease. One means to study miRNA function in animal development is to create mutations. Use of gene-targeting strategies based on ends-out h ...

Here, we detail a protocol to design and introduce sequence-specific cholesterol-conjugated antisense oligonucleotides into mouse organ culture. We review design principles for “antagomirs”, antisense oligos with a cholesterol-moiety modification at the 3′, and present an o ...

MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that regulate gene expression at the posttranscriptional level and play a critical role in many important biological processes and pathological development. In the past few years, miRNAs have been implicated to play an importa ...

Small RNAs (sRNAs) have made a large impact on many recent scientific discoveries. MicroRNAs (miRNAs) are a type of sRNA molecule and, although usually just 20–22 nucleotides in length, they are potent regulators of gene expression. Therefore, identification of miRNAs and profiling their a ...

A variety of physiological processes are associated with changes in microRNA (miRNA) expression. Analysis of miRNA has been applied to study normal physiology as well as diseased states including cancer. One major challenge in miRNA research is to accurately and practically determine ...

Small RNAs (sRNAs) play fundamental roles in modifying the transcriptomes and proteomes of organisms. sRNAs can be classified according to their origin and way of action into classes such as microRNAs, trans-acting siRNAs, heterochromatin siRNAs, Piwi-interacting RNAs, and natural ...

MicroRNAs (miRNAs) are short, about 21 nucleotides in length, non-coding, regulatory RNA molecules representing a new layer in post-transcriptional gene expression regulation. Spatial and temporal analysis of miRNA accumulation by in situ analyses is the prerequisite of unders ...

The detection of microRNAs (miRNAs) in situ presents several technical challenges. Although protocols for mRNA detection by in situ hybridization are well established, the small size of miRNAs makes their localization problematic. To overcome this, digoxygenin-labeled locked n ...

The vascular endothelium was, in the past, considered to be relatively pas- sive, merely acting as a filter between the blood and the vessel wall. It is now clear that endothelial cells actively contribute to the maintenance of vascular homeostasis. Endothelial cells synthesize and secrete ac ...

Cell culture is one of the major tools of cell biologists. It has also become an integral part of the daily routine of most oncology laboratories for the purpose of karyotyping, chemoresistance testing, or basic research. It provides investigators with an opportunity to investigate many cell ...

Human brain neoplasms comprise a highly heterogeneous and biologically diverse group of tumors, the most common and most malignant of which are those of neuroepithelial origin (1) Despite intensive research, little is still understood about the cellular and molecular processes inv ...

Human adipose stromal cells provide an excellent model for studying a variety of metabolic processes in an in vitro system These are normal cells derived from subcutaneous or omental adipose tissue. Under specific culture condition, they will differentiate without replication into c ...