细胞技术

Dental pulp stem cells (DPSCs) can be found within the “cell rich zone” of the dental pulp. Their embryonic origin, from neural crests, explains their multipotency. Up to now, it has been demonstrated that these cells are capable of producing bone tissue, both in vitro and in vivo, as well as a simil-dentin ti ...

Isolation of progenitor cell population is the first step in performing stem cell research. Progenitors isolated using different methods usually exhibit different characteristics and varied differentiation potentials. Here, we describe a method for isolation of the multipo ...

Mesenchymal stem cells (MSC) are currently considered the most promising type of adult stem cells for therapeutic applications, because they can be easily isolated from the bone marrow and other tissues, and manipulated for different applications. The genetic transformation of MSC us ...

Human mesenchymal stromal cells (hMSCs) play a crucial role in tissue engineering and regenerative medicine and thus have important clinical potential for cell-based therapy. However, the limited cell number and the difficulty in detecting these cells in vivo have restricted many hMSC ...

Stem cells are known by their capacity of self-renewal and differentiation into at least one specialized cell type. Mesenchymal stem cells (MSCs) were isolated initially from bone marrow but are now known to exist in any vascularized organ or tissue in adults. MSCs have a great therapeutic poten ...

The hematopoietic stem cells that are present in bone marrow and umbilical cord blood are promising materials for in vitro production of red blood cells (RBCs). In particular, umbilical cord blood cells are likely to be readily available since they are generally discarded after parturition. ...

The study and investigation of cancer stem cells (CSCs) or tumour initiating cells (TICs) have received enormous attention over the 10 years. CSCs are rare, quiescent and capable of self-renewing and maintaining tumour growth and heterogeneity. Better understanding of CSCs will no doubt l ...

Nanolabeling is an invaluable novel technique in biology to detect and characterize different parts of biological systems including microscopic entities, viz., cells inside the living systems. Stem cells (SCs) are multipotent cells with the potential to differentiate into bone, ca ...

The majority of epithelial cells in the distal lung of rodents and humans are quiescent in vivo, yet certain cell populations retain an intrinsic capacity to proliferate and differentiate in response to lung injury or in appropriate culture settings, thus giving them properties of stem/pr ...

The study of adult stem cell populations provides insight into the mechanisms that regulate tissue maintenance in normal physiology and many disease states. With an impressive rate of epithelial renewal driven by a pool of multipotent stem cells, the intestine is a particularly advantag ...

Proliferation in mouse oesophageal epithelial cells is confined to the basal layer of the epithelium. Within this population, it is possible to discriminate different sub-populations using a combination of cell kinetic studies and functional assays. In particular, it is possible to d ...

Although stem cells are commonly isolated by fluorescence-activated cell sorting or magnetic affinity cell sorting, they are very expensive, and they need known markers. However, there is no specific marker for liver stem/progenitor cells (LSPCs). Here, we describe a convenient and eff ...

Hematopoietic stem cells are defined by their ability to self-renew and differentiate through progenitor cell stages into all types of mature blood cells. Gene-targeting studies in mice have demonstrated that many genes are essential for the generation and function of hematopoietic ...

The tubal/uterine lumen is a dynamic environment in which oocytes, eggs, and early embryos are submitted to different forces generated by cilia and peristaltic flow of tubal fluid. The movement of the tubal/uterine fluid, the chemical diversity, and their interaction produce a unique envi ...

The first tissue culture media were developed nearly 150 years ago by Ludwig and Ringer. These were simple salt solutions, which were initially based on the chemical properties of blood serum. The second generation of culture media was developed more than a century later, in the 1970s, aiming to mim ...

Many aspects of the embryo culture environment have been shown to affect embryo development and the subsequent outcomes of human ART. It is now becoming increasingly evident that embryo and later development can be affected by events and conditions that occur before, perhaps long before, the ...

Mineral oil overlay microdrop is commonly used during in vitro fertilization (IVF) procedures. Though mineral oil appears homogeneous, it is an undefined product that can vary in quality. Here, we describe the history, chemistry, processing, and optimal use of mineral oil for IVF and embryo cu ...

Poor laboratory air quality is a known hazard to the culture of human gametes and embryos. Embryologists and chemists have employed analytical methods for identifying and measuring bulk and select air pollutants to assess the risk they pose to the embryo culture system. However, contamina ...

Embryo density is defined as the embryo-to-volume ratio achieved during in vitro culture; in other words, it is the number of embryos in a defined volume of culture medium. The same density can be achieved by manipulating either the number of embryos in a given volume of medium, or manipulating the volume ...

The tension of oxygen measured in the oviducts of several mammals was 5–8.7 %, but this drops in the uterine milieu to