细胞技术

Elastic liposomes have been developed and evaluated as novel topical and transdermal delivery systems. They are similar to conventional liposomes but with the incorporation of an edge activator in the lipid bilayer structure to provide elasticity. Elastic liposomes are applied non ...

Liposomes are widely investigated for their applicability as drug delivery systems. However, the unstable liposomal constitution is one of the greatest limitations, because the liposomes undergo fast elimination after application to the human body. In the presented study, novel a ...

Magnetoliposomes (MLs) consist of nanosized, magnetisable iron oxide cores (magnetite, Fe3O4) which are individually enveloped by a bilayer of phospholipid molecules. To generate these structures, the so-called water-compatible magnetic fluid is first synthesized by co-pr ...

Ultrasound-responsive liposomes are drug-loaded liposomes that contain a small amount of gas (often air). Co-encapsulation of a pharmaceutic along with this gas renders the liposomes acoustically active, allowing for ultrasound imaging as well as controlled release of the conten ...

The following chapter introduces a remote loading procedure for anthracyclines focussing on the well-established drug doxorubicin. The key advantage of remote loading is that it leads to higher drug to lipid ratios and encapsulation efficiencies compared to conventional passive ...

Here, we report methods of preparation for liposome formulations containing lipophilic drugs. In contrast to the encapsulation of water-soluble compounds into the entrapped aqueous volume of a liposome, drugs with lipophilic properties are incorporated into the phospholipid b ...

Structural hemoglobinopathies and thalassemias are congenital hemoglobin (Hb) disorders that cause anemia, morbidity, and mortality resulting from abnormal Hb function. Structurally different normal Hb variants include HbA2, HbF (fetal hemoglobin), and HbA (adult hemog ...

From the clinical perspective, steroids have always held a great deal of interest, since they are highly specific in their function. They do not have a general or systemic effect, but instead regulate specific physiological functions, such as sex differentiation, fetus implantation and gr ...

Urine screenings for drugs of abuse are performed for many reasons, two of the most common being workplace/employment screening and monitoring in drug-dependence treatment centers. In both, the initial screen is commonly an immunoassay. Negative results require no further action. Po ...

Capillary electrophoresis (CE) has several advantages, such as high resolution, speed, and low cost of operation. However, it suffers from two major drawbacks: poor detection limits and matrix effects. Several approaches have been used to overcome these two problems. Here, acetonitrile ...

Antiepileptic drugs vary greatly in chemical structure. Analysis of these compounds by capillary electrophoresis (CE) requires different conditions for each one, or for each group. Acidic drugs can be analyzed easily by capillary zone electrophoresis in borate buffers (1); the neutr ...

Differential activity of genes is one of the major mechanisms underlying a vast array of biological phenomena. Classical genetic approaches (from phenotypes to genes) have proven their exquisite potential for dissection of complex signaling pathways regulating the development ...

Hematopoietic stem cells (HSCs) are rare, self-renewing progenitors that give rise to all lineages of blood cells. HSCs can be found in all hematopoietic organs, from the para-aortic mesoderm (1),(2) and yolk sac (3),(4) in fetuses to the bone marrow (reviewed in ref. 5), blood and spleens of adults.

Human hematopoietic stem cells (HSCs) can be obtained from a variety of hematopoietic tissues, including bone marrow, blood, cord blood, and fetal liver. Various techniques have been used to fractionate hematopoietic cell populations based on differences in size and density, express ...

The hematopoietic system comprises a concatenated series of stem- and transit-progenitor-cell compartments of progressively restricted potentiality and proliferative capacity (1–5). Analysis of hematopoietic regulation in transplantation models and in marrow rege ...

Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including ficoll-density fractionation and presort enric ...

Hematopoietic progenitor and stem cells are believed to lie dormant within the adult bone marrow microenvironment in a state characterized by both mitotic and metabolic quiescence. This state of cell-cycle quiescence has been the focus or target of many studies aimed at identifying cells ...

The hematopoietic progenitors that can be assayed in clonal culture systems represent a continuum of differentiation, which includes multipotential progenitors and very late-committed progenitors with only limited cell-division capabilities (1). The late-committed pr ...

In normal adults, the majority of primitive hematopoietic cells are concentrated in the bone marrow, where they are in contact with a variety of molecules that influence their cell-cycle status, viability, motility, and differentiation. These include components of the extracellular ...

Hematopoietic stem cell (HSC) subsets are defined by the capacity to which their offspring can contribute to the various mature blood-cell lineages. However, the proliferative potential of stem cells is highly dependent on the environment in which they reside, and it is only in retrospect th ...