生物化学技术

TISSUE CULTURE STOCK SOLUTIONS AND MEDIAMS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:Sucrose ........... 10.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3............... 20.0 ml 940 mM (95.00 g/L)NH4NO3............. 20.0 ml 1000 mM (80.04 ...

Tissue ProcessingTissues from the body taken for diagnosis of disease processes must be processed in the histology laboratory to produce microscopic slides that are viewed under the microscope by pathologists. The techniques for processing the tissues, whether biopsies, larger s ...

Processing of Microdissected Tissue for Molecular AnalysisDNA-based Studies These methods were successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objec ...

Processing of Microdissected Tissue for Molecular AnalysisRNA-based Studies This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectiv ...

Immuno-Laser Capture MicrodissectionA: Development of Immuno-LCM Limitation of Microdissection Microdissection of routinely stained or unstained frozen sections has been used successfully to obtain purified cell populations for the analysis of cell-specific gene exp ...

MicrodissectionManual Microdissection This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.About Manu ...

Paraffin Embedding 石蜡加填充剂This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.1. Materials Sakura FineTek V. ...

MicrodissectionOverviewThe Goals of MicrodissectionHuman tissues are comprised of multiple interacting cell populations in a complex three dimensional arrangement with each cellular phenotype determined by a unique profile of mRNA and protein expression. Before micr ...

Low-melt Polyester EmbeddingThis method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.1. Materials 70%, 90%, 99%, 100% e ...

70% Ethanol Fixationfor Recovery of DNA, RNA, and ProteinThis method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study.Th ...

4% PARAFORMALDEHYDE/PBS 1. Measure 100ml Phosphate Buffered Saline (PBS) into a measuring cylinder. Pour into the conical flask containing 4g of paraformaldehyde. Cover with parafilm and transfer to the fume hood: thoroughly shake - take care not to splash paraformaldehyde about - it is a rap ...

激光显微切割 Laser Microdissection protocolsDiscussion of Laser Microdissection ProjectsSince ideal tissue preparation parameters for mesenchymal tissues and epithelial tissues differ and because considerations of fixation, protocols may vary depending upon the ulti ...

GIEMSA FOR Helicobacter pylori METHOD 1. Sections to water. 2. Dilute Giemsa 1:20 with distilled water and add 2 drops of concentrated acetic acid. stain for 30 minutes 3. Wash in water. 4. Dehydrate, clear . Mount sections in DPX RESULTS --Helocobacter blue/grey --Background pink/pale blue --Nuclei ...

POLY-L-LYSINE COATED SLIDES SOLUTIONS --5% Chromic acid --0.01% Poly-L-Lysine (PLL) - made from a dry protein, with distilled water. (100mg/1 litre) This procedure can be carried out on either a ski or carousel staining machine. SKI STAINING MACHINE 1. Remove the new slides from box. 2. Clip them individ ...

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUEConstance Cepkundefined, Elizabeth Ryder1, Donna M. Fekete2, and Suzanne BruhnundefinedHarvard Medical School and the Howard Hughes Medical InstituteBoston, MA 02115 Current Addresses:1Department of Biology a ...

Rapid Processing of Fresh Tissue into ParaffinFresh tissue

Glutaraldehyde is an extremely toxic substance harmful by ingestion and inhalation. Extremely irritating to eyes. Prolonged skin contact can cause dermatitis and sensitisation.SPILLAGE DISPOSALWear appropriate protective clothingIf local regulations permit, mop up w ...

1. Sections to distilled water. 2. Stain in Fouchets reagent 5 minutes. 3. Rinse in distilled water. 4. Counterstain with van gieson 5 minutes. 5. Rinse quickly in absolute alcohol. 6. Dehydrate, clear . Mount sections in DPX Results --Bile pigments Blue/Green --Muscle Yellow --Collagen Red SOLUTIONS ...

嗜银颗粒染色 Stain for argyrophil granules ：GRIMELIUS1. Sections to water. 2. Treat sections at 65ªc with silver solution for 3 hours 3. Drain silver solution from slide. 4. Treat with freshly prepared reducing solution at 45ªc for 1 minute. 5. Wash in distilled water 6. Repeat step 4 until the desired result is ...

石蜡切片 Parrafin SectioningSolutions 20% Paraformaldehyde/4% Paraformaldehyde-PBS200 g paraformaldehyde1 ml 10N NaOHup to 1 liter with Q, heat to 65° to dissolve, aliquote and store at -20°Mix 100 ml 20% Paraformaldehyde with 50 ml 10X PBS and bring up to 500 ml with Q filter, and store at 4° for up to 2 weeksP ...