FOUCHET METHOD stain for bile
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1. Sections to distilled water.
2. Stain in Fouchets reagent 5 minutes.
3. Rinse in distilled water.
4. Counterstain with van gieson 5 minutes.
5. Rinse quickly in absolute alcohol.
6. Dehydrate, clear . Mount sections in DPX
Results
--Bile pigments Blue/Green
--Muscle Yellow
--Collagen Red
SOLUTIONS
Fouchet reagent
--25% aqueous trichloroacetic acid 25ml
--10% aqueous ferric chloride 25ml
Van Gieson Solution
--see "Elastic Van Gieson" Method.
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