ECACC RPMI 8866细胞

欧洲认证细胞培养物保藏中心 (ECACC) 是英国公共卫生的四个培养物保藏中心之一。 我们提供经过验证和质量控制的细胞系、核酸和诱导多能干细胞 (iPSC)。
ECACC 成立于 1985 年，是一家为研究界提供服务的细胞培养收集机构，并为欧洲提供国际保藏机构认可的专利保藏机构。
如此多样化的馆藏的开发和维护不可避免地产生了高水平的专业知识，再加上英国公共卫生部门的支持，使 ECACC 能够将自己定位为细胞培养各个方面的专业知识中心。
我们提供全面的细胞培养服务，包括细胞系认证和培训课程，并多元化进入新产品领域，例如从细胞系和 iPSC 中提取的高质量基因组 DNA。

以国内使用较多的细胞系#95041316，RPMI 8866 为例，产品详情如下：
ECACC General Cell Collection: RPMI 8866

Supplied by:	European Collection of Authenticated Cell Cultures (ECACC)
Culture Type:	Cell line
Collection:	ECACC General Collection
Catalogue No.:	95041316
Cell Line Name:	RPMI 8866
Citation Guidance:	If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 8866 (ECACC 95041316)
Keywords:	Human B lymphocyte
Cell Line Description:	RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).
Species:	Human
Tissue of Origin:	blood
CellType:	Lymphoblastoid
Growth Mode:	Suspension
DNA Profile:	STR-PCR Data: Amelogenin: X CSF1PO: 11 D13S317: 10,14 D16S539: 11 D5S818: 12 D7S820: 11,12 THO1: 9.3 TPOX: 8,11 vWA: 14,16
Karyotype:	Not specified
Biosafety Information:	Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises. Hyperlinks to MSDS documents: Frozen cell cultures Material Safety Data Sheet Growing cell cultures Material Safety Data Sheet Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine:	To keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37 C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.
Culture Medium:	RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor:	Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507
Originator:	Yes
Country:	USA
References:	J Immunol 1967;99:839; J Biol Chem 1974;249:6812
Additional Bibliography:	Not specified
Patents:	None specified by Depositor
Release Conditions:	No

Available Formats: Frozen
细胞图片：



ECACC细胞库中保藏的细胞系类型有：
General Cell Collection
More than 1100 different cell lines originating from over 45 different species and a wide variety of tissues. Search, browse or look through our cell lines grouped according to a particular characteristic or area of study: 

• Colorectal Cell Lines
• Neurobiology Cell Lines
• Oesophageal Cell Lines
• Serum Free Cell Lines

 
Induced Pluripotent Stem Cell (iPSC) Lines
A range of human induced pluripotent stem cell (iPSC) lines derived from individuals with a known disease status or described as normal for use as controls.  The cell lines available are distributed by ECACC on behalf of the European Bank of induced pluripotent Stem Cells (EBiSC) or the Human Induced Pluripotent Stem Cells Initiative (HipSci).   
 
SCREENflex GPCR Cell Lines
A range of G-protein coupled receptor (GPCR) cell lines available as single seed vials or as Frozen, Assay Ready reagent.
 
Hybridoma Collection
Over 400 monoclonal antibody-secreting hybridomas with a diverse range of antigen specificities.
 
Chromosomal Abnormality Collection
A subset of samples from the Human Genetic Collection represented by cell lines (the majority are B-lymphoblastoid cell lines) and peripheral blood leukocyte samples derived from individuals determined to carry chromosomal abnormalities.
 
HLA-Typed Collection
A specialised collection of over 430 reference B-lymphoblastoid cell lines, originating from laboratories all over the world, for which Human Leukocyte Antigen (HLA) typing data is available.
 
Human Random Control Collection
A collection of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland.
新细胞系：

Catalogue number	Cell line name	Keywords
16110902	N14A	Pseudomyxoma peritonei, PMP, appendicitus, appendiceal origin, appendix
16110903	N15A	Pseudomyxoma peritonei, PMP, appendicitus, appendiceal origin, appendix

 

Catalogue number	Cell line name	Keywords
17100525	HeLa mCherry-Histone H2B EGFP-Alpha Tubulin	EGFP, Human Histone H2B, mCherry, Alpha Tubulin, fusion protein, H2B-mCherry, EGFP-Alpha Tubulin

 

Catalogue number

Cell line name

Keywords

16022517

MCF7/AnaR-2

Breast cancer, MCF7, Anastrozole resistance, aromatase inhibitors, ER+

16022520

MCF7/ExeR-1

Breast cancer, Exemestane resistance, aromatase inhibitor, MCF7, ER+

16022521

MCF7/ExeR-2

Breast cancer, Exemestane resistance, aromatase inhibitors

16022522

MCF7/ExeR-3

Breast cancer, Exemestane resistance, Aromatase inhibitors

Catalogue number

Cell line name

Keywords

15121704

HT29-5FU resistant (1 microMolar)

HT29-5FU, 5-Fluorouracil-resistance, goblet cells, gastric mucins. dome-forming cells, 5FU

15121705

HT29-MTX resistant (1 microMolar)

HT29-MTX, methotrexate-resistance, goblet cells, gastric mucins, MTX

15121709

HT29-5FU resistant (10 microMolar)

HT29-5FU, 5-Fluorouracil-resistance, goblet cells, gastric mucins, 5FU, 5-fluorouracil, 5-FU

15121710

HT29-MTX resistant (100 microMolar)

Colon carcinoma, drug resistance, methotrexate, HT29-MTX, methotrexate-resistance, goblet cells, gastric mucins

15121711

HT29-MTX resistant (10 microMolar)

HT29-MTX, methotrexate-resistance, goblet cells, gastric mucins

Catalogue number

Cell line name

Keywords

19061801

Rev-Erb alpha (NR1D1)

Nuclear Receptor subfamily 1, group D member 1, (NR1D1), Hynridoma (6F05-2), mouse mAb

Catalogue number

Cell line name

Keywords

19031501

KT15

T cell clone, C6, H-2KK restricted antigen specific CTL, monoclonal antibody, mouse

Catalogue number

Cell line name

Keywords

19022601

Aag2-AF5

Aedes aegypti, clonal cell line

Catalogue number

Cell line name

Keywords

19022602

Aag2-AF319

Aedes aegypti-derived clonal cell line, Dcr2 knockout, RNAi deficiency

Catalogue number

Cell line name

Keywords

16022503

MCF7/164R-4

Breast cancer, fulvestrant resistance, ER alpha

16022505

MCF7/164R-7

Breast cancer

16022510

MCF7/TAMR-8

Breast cancer, tamoxifen-resistant

16022516

MCF7/AnaR-1

Breast cancer, MCF7, Anastrozole resistance

16022518

MCF7/AnaR-3

Breast cancer, MCF7, Anastrozole resistance

16022525

MCF7/LetR-2

Breast cancer, Letrozole resistant

16022526

MCF7/LetR-3

Breast cancer, Letrozole resistant

16022528

MCF7/TAMR-4

Human, Breast, Cancer, Oestrogen receptor, Tamoxifen resistant

Catalogue number

Cell line name

Keywords

17100230

SG01

Subglottic stenosis, cancer, inflammation, immortalised, cell line

  

Catalogue number	Cell line name	Keywords
17100502	Wilms’ Tumor 17.94	Wilms’ Tumor, cell line, anaplastic

 

Catalogue number	Cell line name	Keywords
17100529	HeLa EGFP-Histone H2B	PP6 catalytic activity, EGFP, EGFP-tagged histone H2B

 

Catalogue number	Cell line name	Keywords
17100531	HEK293-A mRFP-ATG9	Autophagy, Cancer, Cell Signalling, Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism
17100532	HEK293 GFP-WIPI2	Cancer, neurodegeneration, autophagy
17100533	HEK293 GFP-WIPI1	Cancer, neurodegeneration, autophagy

 

Catalogue number	Cell line name	Keywords
17100534	CIOV1	Ovarian cancer, HGSOC
	CIOV2	*Not yet available. Coming soon to ECACC*
17100536	CIOV3	High grade serous ovarian cancer, TP53 mutation, BRCA1 and BRCA2 mutations, PTEN, RB1.

 

Catalogue number	Cell line name	Keywords
15121701	HT29-5F7	HT29-5F7, 5-fluorouracil and methotrexate-resistance, enterocytic cells, MUC1 mucin
15121702	HT29-5F12	HT29-5F12, 5-fluorouracil -resistance, enterocytic cells, MUC2 mucin
15121706	HT29-5M21	HT29-5M21, methotrexate-resistance, clone, MUC5AC and MUC5B
15121707	HT29-5M12	HT29-5M12, 5-fluorouraci, methotrexate-resistance, clone, mucin, MUC5B

 

Catalogue number	Cell line name	Disease status
77650823	HPSI1116i-zehh_3	Rare genetic neurological disorder
77650824	HPSI1116i-zehh_4	Rare genetic neurological disorder
77650826	HPSI1016i-eoxu_2	Rare genetic neurological disorder
77650827	HPSI1016i-eoxu_6	Rare genetic neurological disorder
77650793	HPSI1016i-livl_2	Rare genetic neurological disorder
77650794	HPSI1016i-livl_5	Rare genetic neurological disorder
77650795	HPSI1016i-riwg_2	Rare genetic neurological disorder
77650796	HPSI1016i-riwg_8	Rare genetic neurological disorder

 

Catalogue number	Cell line name	Disease Status
77650803	HPSI1014i-xejd_1	Normal
77650633	HPSI0616i-biln_1	Hereditary spastic paraplegia
77650800	HPSI0115i-rehy_2	Normal
77650479	HPSI0416i-mefc_1	Usher syndrome and congenital eye defects
77650639	HPSI0416i-mapx_1	Alport syndrome
77650542	HPSI0516i-oadp_5	Kabuki syndrome
77650653	HPSI0616i-giql_1	Bleeding and platelet disorders
77650858	HPSI0115i-payf_1	Normal
77650655	HPSI0616i-gayk_3	Bleeding and platelet disorders

 

Catalogue number	Cell line name	Keywords
16012201	C-Raf KI D486A	Knock in, oncogenic mutant
16012205	MEF Raf1 FF/FF KI	Knock-in, FF/FF Raf1 (Y340F-Y341F) mutant, inactivation
16012209	MEF RAF1 KO	Knock-out, RAF1, mutant, inactivation

 

Catalogue number	Cell line name	Keywords
16022507	T47D-182R1	Breast cancer, fulvestrant resistant
16022508	T47D-182R2	Breast cancer, fulvestrant resistant
16022510	MCF7/TAMR-8	see December 2020
16022511	T47D/S5	Breast cancer, control for fulvestrant-resistant T47D cell lines
16022528	MCF7/TAMR-4	see December 2020
16022516	MCF7/AnaR-1	see December 2020
16022518	MCF7/AnaR-3	see December 2020
16022520	MCF7/ExeR-1	see December 2020
16022522	MCF7/ExeR-3	see December 2020
16022526	MCF7/LetR-3	see December 2020

 
 

Catalogue number	Cell line name	Keywords
17011201	17011201 HCT 116 p300 KO [D10]	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011211	HCT 116 p300 KO Rescue	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011214	HCT 116 p300 WT	p300 Knock-Out,colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011215	HCT 116 p300 KO [F2]	p300 Knock-Out,colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011216	HCT 116 p300 KO [F5]	p300 Knock-Out,colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration

 

Catalogue No.	Cell Line Name	Keywords
16022512	T47D/S2	Human, Breast, Cancer, Oestrogen receptor
16022509	MCF7/TAMR-7	Breast cancer, tamoxifen-resistant
16022513	T47D/TR-1	Breast carcinoma, tamoxifen resistant, oestrogen receptor
16022523	MCF7/ExeR-4	Breast cancer, Exemestane resistance
16022524	MCF7/LetR-1	Breast cancer, Letrozole resistant
16022519	MCF7/AnaR-4	Breast cancer, MCF7, Anastrozole resistance
16022501	MCF7/S0.5	Human, Breast, Cancer, Oestrogen receptor, MCF7
16022506	MCF7/182R-6	Breast cancer, fulvestrant resistant
Catalogue number	Cell line name	Keywords
15042901	CHO-K1.Cl6	CHO-K1, FcγRIIIa , Fc gamma receptor 3A, 158V, allotype, human IgG , IgG binding
15042902	CHO-K1.Cl7	CHO-K1, FcγRIIIa , Fc gamma receptor 3A, 158F, allotype, human IgG , IgG binding
15042903	CHO-K1.Cl-0204	CHO-K1, FcγRIIa , Fc gamma receptor 2A, 131R, allotype
15042905	CHO-K1.Cl-0205	CHO-K1, FcγRIIa , Fc gamma receptor 2A, 131H, allotype, human IgG , IgG binding
15042907	CHO-K1.Cl-0206	CHO-K1, FcγRIIb , Fc gamma receptor 2b
15042908	CHO-K1.Cl-0235	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Pan troglodytes, chimpanzee
15042909	CHO-K1.Cl-0236 / CHO-K1.Cl-0239	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Cercocebus torquatus, red-crowned mangabey, papio anubis, olive baboon
15042910	CHO-K1.Cl-0237	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Macaca fascicularis (Cynomolgus macaque)
15042911	CHO-K1.Cl-0238	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Macaca mulatta (Rhesus macaque)
15042912	CHO-K1.Cl-0270	CHO-K1, non-human primate FcγRIIb , Fc gamma receptor 2b, Macaca mulatta, Rhesus macaque, Macaca fascicularis, Cynomolgus macaque

 
 

Catalogue No.	Cell line name	ULK 1 and 2 status	Immortalisation method
14050808	ULK1/2 WT MEF (SIM)	Wild type	Spontaneously immortalised
14050804	ULK1/2 WT MEF (SV40)	Wild type	SV40-immortalised
14050806	ULK1 KO MEF (SIM)	ULK1 knock-out	Spontaneously immortalised
14050807	ULK1 KO MEF (SV40)	ULK1 knock-out	SV40-immortalised
14050810	ULK2 KO MEF (SIM)	ULK2 knock-out	Spontaneously immortalised
14050805	ULK2 KO MEF (SV40)	ULK2 knock-out	SV40-immortalised
14050803	MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)	Double knock-out	Spontaneously immortalised
14050802	MEF Ulk1 -/- Ulk2 -/- (DKO) (SV40)	Double knock-out	SV40-immortalised