相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C
- 保质期:
12个月
- 英文名:
ViaFluor® 405 Live Cell Microtubule Stain 70064-T
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
ViaFluor® Live Cell Microtubule Stains are cell-permeant TAXOL® (paclitaxel) probes for imaging the microtubule cytoskeleton in live cells. They are simple, rapid and sensitive stains that can be imaged without a wash step.
- Cell-permeant fluorescent microtubule probes
- Rapid, sensitive, no-wash stains for live cells
- Available with blue, green, or far-red fluorescence
- ViaFluor® 647 dye compatible with SIM or STED
TAXOL® binds to polymerized tubulin and stabilizes microtubules, resulting in inhibition of mitosis. However, fluorescent TAXOL® compounds like ViaFluor® Microtubule Stains are less disruptive of microtubule dynamics and cell division, presumably due to lower binding affinity of the fluorescent probe compared to TAXOL® itself. The probes can be used to stain cells for as long as 48 hours to several days. However, the probes may show cytotoxicity and cell cycle arrest with longer incubation times. The stains are supplied as a 1000X stock solutions in DMSO, and include a vial of 100 mM verapamil, an efflux pump inhibitor that may improve probe retention and staining in some cell types.
ViaFluor® Live Cell Microtubule Stains cannot be fixed after staining, and cannot be used with fixed cells or tissues. The probes are not predicted to bind bacterial or yeast microtubules. Learn more about our other Fluorescent Cellular Stains for nucleus, mitochondria, lysosomes, or see our selection of Microbiology Stains & Kits.
ViaFluor® Live Cell Microtubule Stains
| Dye | Ex/Em | Catalog no. | Size (1000X in DMSO)* |
Features |
|---|---|---|---|---|
| ViaFluor® 405 | 408/452 nm | 70064-T | 10 uL | Blue fluorescence for the DAPI channel |
| 70064 | 50 uL | |||
| ViaFluor® 488 | 500/515 nm | 70062-T | 10 uL | Green fluorescence for the FITC channel |
| 70062 | 50 uL | |||
| ViaFluor® 647 | 650/675 nm | 70063-T | 10 uL | Far-red fluorescence for the Cy®5 channel Compatible with SIM or STED |
| 70063 | 50 uL |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
SIINFEKL 6 - 7015 - 001 4) 实验数据 -高染色强度:使用可逆的MHC I STREPTAMERS®染色抗原特异性 (CD8 + T) 细胞 STREPTAMERS® 的高染色强度,可以最佳化的检测到小型T细胞群体。对 CMV-specific CD8+ T 细胞使用 HLA-A* 0201 CMV pp65495 - 503 Streptamer® 进行体外染色 (右),并与常规多聚体 (左) 进行染色
Vybrant® DyeCycle Green and Orange Stains
Stability Vybrant® DyeCycle™ Green stain Vybrant® DyeCycle™ Orange stain 400 μL 5 mM solution in dimethyl sulfoxide (DMSO
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
1) Resuspend the Dynabeads Mouse T-Activator CD3/CD28 in the vial. 2) Transfer the desired volume of Dynabeads to a tube. 3) Add an equal volume of Buffer, or at least 1 ml, and mix (vortex for 5 seconds











