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- Biotium
- 美国
- 90049-10mg
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
6个月
- 英文名：
Cyanine 750 SE
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
- 保存条件：
-20°C避光
Biotium offers high quality amine-reactive succinimidyl ester (SE, or NHS-Ester) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555 (Cy®3), Cyanine 647 (Cy®5), Cyanine 680 (Cy®5.5), and Cyanine 750 (Cy®7) as high quality succinimidyl esters. Also see our CF® Dye Succinimidyl Esters, available with more than 20 bright and photostable CF® Dyes.
Cyanine 555
Cyanine 555 is structurally identical to Cy®3 from GE Healthcare.
- λEx/λEm (water) = 555/565 nm
- Dark red solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C41H56N4O10S2
- MW: 829
Cyanine 647
Cyanine 647 is structurally identical to Cy®5 from GE Healthcare.
- λEx/λEm (water) = 650/665 nm
- Dark blue solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C43H58N4O10S2
- MW: 855
Cyanine 680
Cyanine 680 is structurally identical to Cy®5.5 from GE Healthcare.
- λEx/λEm (water) = 681/698 nm
- Dark blue solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C63H92N6O16S4
- MW: 1318
Cyanine 750
Cyanine 750 is structurally identical to Cy®7 from GE Healthcare.
- λEx/λEm = 755/777 nm
- Dark blue solid soluble in DMF and DMSO
- Store at -20°C and protect from moisture and light
- C45H60N4O10S2
- MW: 881
Cy3, Cy5, Cy5.5, and Cy7 are registered trademarks of GE Healthcare.
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文献和实验Cyanine Dye Purification Protocol
) 20 ml ddH2 O, 0.22 mm filtered 15 mM TEAA (this can be substitutied with 0.750 mL HSB and 49.25 mL ddH2 O, 0.22 mm filtered, to produce 50 mL) Sep-Pak cartridges, C18 (Waters #20515); 1 column per cy dye. Can be reused twice
SE 活性部分远低于50%（AlexaFluor488微量标记试剂盒产品信息，Invitrogen）。相反，所有Biotium 的CF染料具有相对稳定的胺活性的SE基团型，这比大部分AlexaFluor 染料的SE更耐水解。总体来说，CF染料SE产品一贯的给出更高的标记效率，提供给用户更好的使用价值。选择方案：蓝色（350-450nm处激发） CF 350、Alexa Fluor 350、AMCA等----亮蓝和紫外光激发CF350是类似于Alexa Fluor 350和传统荧光染料AMCA的蓝色
DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
thread of DNA with a closed hooked pasteur pipet and rinse gently in 70% EtOH - DNA is dried and dissolved in appropriate volume of TE buffer - dissolve the DNA o/n at 4℃ on rotor SE buffer (10X) : 750 mM NaCl pH=8 250 mM EDTA
