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文献和实验Fabrication of Carbohydrate Surfaces by Using Non-derivatised Oligosaccharides
. A thiol-functionalised surface was derivatised with a heterobifunctional cross-linker to yield a hydrazide surface. This was treated with oligosaccharides, derived from keratan sulphate, chondroitin sulphate, and heparin that possess a reducing end
Site-directed hydroxy radical mapping of nucleosome positions in vitro
with the cysteine thiol and there is only a single cysteine (H3 Cys110) in the four Xenopus laevis core histones. The equivalent residue is an alanine in yeast and the alanine can be mutated to cysteine without any known biochemical effect. A popular reagent
) and GST-FRB and (His)6-FKBP12 were produced recombinantly in Escherichia coli. IKBalpha and (His)6-FKBP12 were labeled with Cy3 and Cy5, respectively, using monofunctional reactive dye from Amersham Pharmacia Biotech (Newark, NJ) and following
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