- ￥1200
- Biorigin
- BN41481R
- 2025年08月08日
- WB,IHC-P,FCM,
- Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,
- 应用范围：
WB,IHC-P,FCM,
- 抗体英文名：
Caspase-3
- 规格：
50ul
| 英文名称 | Caspase-3 |
| 中文名称 | 活化半胱胺酸蛋白酶蛋白-3抗体 |
| 别 名 | Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Rabbit, (predicted: Dog, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit:1-100/277 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Reacts with Caspase-3 subunit p17 and precursor. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Subcellular Location: Cytoplasm. Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Post-translational modifications: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Similarity: Belongs to the peptidase C14A family. SWISS: P42574 Gene ID: 836 Database links:
Entrez Gene: 836 Human Entrez Gene: 12367 Mouse Entrez Gene: 100008840 Rabbit Omim: 600636 Human SwissProt: P42574 Human SwissProt: P70677 Mouse SwissProt: Q8MJC3 Rabbit Unigene: 141125 Human Unigene: 34405 Mouse Unigene: 10562 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Caspase3广泛分布于各种不同类型的细胞中，是Caspase家族中最重要的凋亡执行者之一，激活的Caspase-3能使许多与细胞结构、细胞周期及DNA修复等相关蛋白或激酶失活，从而使细胞凋亡. |
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文献和实验天冬氨酸特异性半胱氨酸蛋白酶(cysteine-containing aspartate-specific proteases，c
caspase是一类蛋白 酶家族，成员较多，在人类，已经鉴定了10种不同的caspase。各种caspase都富含半胱氨酸，它们被激活后，能够在靶蛋白 的特异天冬氨酸残基部位进行切割。在正常的细胞内，每一种caspase都是以非活性状态存在的，这种非活性的caspase称作酶原（zymogen），它是酶的非活性前体，其肽链比有活性时长一些，将多出的部分切除，就转变成有活性的caspase。有两类caspase，一类是起始者（initiators）,另一类是执行
天冬氨酸特异性半胱氨酸蛋白酶(cysteine-containing aspartate-specific proteases，caspase)
caspase是一类蛋白酶家族，成员较多，在人类，已经鉴定了10种不同的caspase。各种caspase都富含半胱氨酸，它们被激活后，能够在靶蛋白的特异天冬氨酸残基部位进行切割。在正常的细胞内，每一种caspase都是以非活性状态存在的，这种非活性的caspase称作酶原（zymogen），它是酶的非活性前体，其肽链比有活性时长一些，将多出的部分切除，就转变成有活性的caspase。有两类caspase，一类是起始者（initiators）,另一类是执行者（executioners
网络 二、C4分子 C4是经典激活途径中第二个被活化的补体成分，分子量约为210kDa，由α（90kDa）、β（78kDa）及γ（33kDa）三条肽链借二硫键连接组成（图5-4）C4的分子结构较为特殊，其α链中含有一个在半胱氨酸和谷氨酸残其间形成的内硫酯键。α链的N端有C1s丝氨酸蛋白酶的作用点。当C1s将C4α链的精氨酸-丙氨酸键
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