- ￥2200
- Biorigin
- BN41090R
- 2025年08月04日
- WB,
- Human,Mouse,Rat,
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
Human,Mouse,Rat,
- 应用范围：
WB,
- 抗体英文名：
phospho-CK18 (Ser60)
- 规格：
100ul
| 英文名称 | phospho-CK18 (Ser60) |
| 中文名称 | 磷酸化细胞角蛋白18抗体 |
| 别 名 | Cytokeratin 18 (phospho S60); p-Cytokeratin 18 (phospho S60); Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; K 18; K18; K1C18_HUMAN; Keratin 18; Keratin D; Keratin type I cytoskeletal 18; keratin, type I cytoskeletal 18; Keratin-18; Keratin18; Kerd; KRT18. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 48kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CK18 around the phosphorylation site of Ser60:MG(p-S)GG |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. Function: When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. Subunit: Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8. Interacts with the thrombin-antithrombin complex (By similarity). Interacts with PNN, HCV core protein and mutated CFTR. Interacts with YWHAE, YWHAH and YWHAZ only when phosphorylated. Interacts with DNAJB6, TCHP and TRADD. Subcellular Location: Cytoplasm, perinuclear region. Nucleus, nucleolus. Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. Post-translational modifications: Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7. O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue. DISEASE: Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. Similarity: Belongs to the intermediate filament family. SWISS: P05783 Gene ID: 3875 Database links: Entrez Gene: 3875 Human Entrez Gene: 16668 Mouse Omim: 148070 Human SwissProt: P05783 Human SwissProt: P05784 Mouse Unigene: 406013 Human Unigene: 22479 Mouse Unigene: 103924 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 结构蛋白（Structural Proteins）常用于肿瘤细胞的分化、增殖及转移方面的研究。 |
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文献和实验memoday 最近在做一个信号通路的下游磷酸化水平检测，但是做western没有看到任何条带,包括p-ERK,p85。麻烦各位战友帮我分析一下可能原因吧： 1.293T细胞转染质粒（带外源基因），24h 2.无血清培养18h 3.配体（外源基因是该配体的膜受体）激活，37度，10min 4.PLB（promega公司）裂解细胞，做western，抗体是SANTA CRUZ公司的p-ERK (Tyr 204)，以及cell
Cell Metab：浙大吕志民团队揭示肿瘤细胞 Warburg 效应促进肿瘤免疫逃逸
2 从线粒体外膜脱落，进入细胞浆中。在细胞浆中，HK2 结合 NF-κB 的抑制因子 IκBα。 重要的是，HK2 发挥了不依赖于其经典代谢功能的新功能，即作为一个蛋白激酶磷酸化 IκBα 的 Thr 291 位点。该磷酸化促进了蛋白酶 μ-calpain 与 IκBα 的结合并进一步降解 IκBα，进而使转录因子 NF-κB 入核，最终促进了 PD-L1 的表达并导致了肿瘤的免疫逃逸。作者还发现，使用己糖激酶的抑制剂与 PD-1 抗体联用治疗小鼠胶质瘤，可以显著提升 PD-1 抗体的治疗
两者没有帮助。但是，使用不同表型角蛋白的抗体，可能对区分两者有一定价值。当然，通过使用针对其它非中间丝抗体在上述鉴别诊断中也会有所帮助。 3．有限特异性的角蛋白单克隆抗体本组抗体能通过对富含细胞骨架标本的凝胶电泳免疫反应，识别非共有的角蛋白表位，但并不一定说明这些单克隆抗体能通过组织学方法成功测定角蛋白的免疫表型。因而还不能肯定这些单克隆抗体对上皮组织肿瘤的进一步分类具有价值，这里不作一一介绍。但是，对已明确知道其免疫反应类型的某些抗体，则可予以使用。比如，对不能识别角蛋白18
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