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brdu
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brdu
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文献和实验Labelling Drosophila pupae with BrdU
Labelling Drosophila pupae with BrdU ITO Kei, 1992 October COLLECTING STAGED PUPAE 1. Prepare a couple of bottles containing late 3rd instar larvae, which wander on the botlle wall (110-120 hours after egg laying at 25 C
Brdu免疫组织化学染色分析 BrdU incorporation assay
Enzyme Immunostaining for BrdU: Wash frozen sections with PBS 2x. Put them in 0.1% Pepsin in 0.1N HCL (in PBS) at 37C for 50min. Then in 0.3% hydrogen peroxide in PBS, 30min. Then rinse with PBS 3x.
Detection of BrdU Incorporation in DNA Synthesizing Cells
Detection of BrdU Incorporation in DNA Synthesizing Cells NOTE: Bromodeoxyuridine is a known carcinogen. Propidium iodine (PI) is known to be toxic and carcinogenic
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