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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
iFluor™ 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide*
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100 Slides
关键词：iFluor 488 Styramide * Alexa Fluor 488酪胺的优异替代品*详细如下：
Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor™ dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor™ dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA radicals have much higher reactivity than tyramide radicals, making the PSA system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor™ 488 Styramide is a superior replacement for Alexa Fluor 488 tyramide or other spectrally similar fluorescent tyramide conjugates or TSA reagents.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-2559 | PerCP [Peridinin-Chlorophyll-Protein Complex] | 1mg |
| AAT-2570 | ReadiUse™ Preactivated APC-iFluor™ 700 Tandem | 1mg |
| AAT-2571 | ReadiUse™ Preactivated APC-iFluor™ 750 Tandem | 1mg |
| AAT-2577 | ReadiUse™ Preactivated PE-iFluor™ 647 Tandem | 1mg |
| AAT-2578 | ReadiUse™ Preactivated PE-iFluor™ 750 Tandem | 1mg |
| AAT-3001 | Biotin *CAS 58-85-5* | 1g |
| AAT-3002 | Biotin, succinimidyl ester *CAS 35013-72-0* | 100mg |
| AAT-3003 | Biotin ethylenediamine *CAS 216299-38-6* | 10mg |
| AAT-3004 | Biotin cadaverine | 25mg |
| AAT-3005 | Biotin C2 maleimide | 25mg |
| AAT-3006 | Biotin-4-fluorescein *CAS 1032732-74-3* | 5mg |
| AAT-3007 | Biotin hydrazide *CAS 66640-86-6* | 25mg |
| AAT-3009 | Biotin-X NTA [Biotin-X nitrilotriacetic acid, potassium salt] *CAS 856661-92-2* | 1mg |
| AAT-3010 | Biotin-X, succinimidyl ester *CAS 72040-63-2* | 25mg |
| AAT-3014 | Biotin PEG2 amine *CAS 138529-46-1* | 5mg |
| AAT-3015 | Biotin PEG2 maleimide *CAS 305372-39-8* | 5mg |
| AAT-3016 | Biotin PEG2 succinimidyl ester | 25mg |
| AAT-3017 | Fluorescein biotin | 5mg |
| AAT-3019 | Biotin-PEG3-azide *CAS 945633-30-7* | 5mg |
| AAT-3020 | Biotin Azide | 5mg |
| AAT-3021 | Biotin Alkyne *CAS 1006592-45-5* | 5mg |
| AAT-3022 | Biotin PEG4 succinimidyl ester | 25mg |
| AAT-3050 | ReadiView™ biotin acid | 25mg |
| AAT-3053 | ReadiView™ biotin amine | 5mg |
| AAT-3055 | ReadiView™ biotin hydrazide | 5mg |
| AAT-3058 | ReadiView™ biotin maleimide | 5mg |
| AAT-3059 | ReadiView™ biotin succinimidyl ester | 5mg |
| AAT-3080 | Biocytin *CAS 576-19-2* | 100mg |
| AAT-3085 | Biocytin C2 maleimide | 5mg |
| AAT-3086 | Biocytin hydrazide *CAS 102743-85-1* | 25mg |
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文献和实验技术原理与方法｜超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色（Alexa Fluor405，蓝色）；CB1 染色（Alexa Fluor488，绿色）；VGluT3 染色（Cy3，红色）；DGLα染色（Alexa Fluor647，白色）。 大麻素合成酶 DGLα（白色）和大麻素受体 CB1（绿色）聚集在小鼠
偶联物，最大限度的提高了信噪比。图2. CF染料的超强特异性；由于AF染料制备过程中含有大量带负电的磺化基团，容易非特异性结合正电基团，请看AF790 Dye与CF790的条带特异性对比。3. 优异的标记效率生物偶联后的活性染料一般很容易水解，会在运输，操作及储存中带来诸多不便，最终致染料结合效率低下。像Alexa Fluor® dyes,DyLight? dyes and IRDyes®等严重的磺化作用有较强的吸湿性，恶化水解作用等问题。例如： AlexaFluor® 488 在应用时琥珀酰亚胺脂酯
，Alexa Fluor 488）和γH2AX（红色，Alexa Fluor 594）。通道合并图像显示 CDH4 和γH2AX 的共定位。
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