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文献和实验Ampicillin (50mg/ml) Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H2 0. Store 1 ml aliquots at 20o C in common freezer for stock solutions 上一篇:PREPARATION
Miniprep Kit Procedure This figure shows the general In-Fusion BioBrick assembly scheme for BioBricks on pSB1A2 plasmids. See details below. Part A (blue) and Part B (red) are on pSB1A2 plasmids encoding ampicillin resistance. Primers described
1. suspend a single plaque in 1 ml PSB 2. adsorb 10 min at 37°C: 0.1 ml eluted phage*/0.1 ml MgCa/0.1 ml saturated K802 culture grown in NZY broth/0.2% maltoseuse 10 ml of this to inoculate 2.5 ml NZY, shake ovn at 37°C or until lysed 3. cool
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