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文献和实验Oxalyticase Dissolve in 0.1 M NaCl, 0.02% Na azide, 50% glcyerole Store aliquots (1 ml) at 20o C in common freezer for stock solutions 上一篇:ALPHA FACTOR ARREST AND RELEASE 下一篇:GLASS BEADS
Reducing Sample Complexity by RP-HPLC: Beyond the Tip of the Protein Expression Iceberg
-phase high-performance liquid chromatography (RP-HPLC), separating proteins according to their hydrophobicity. This effectively introduces a third separation dimension, increasing the spatial resolution of the experiment
Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps
, we describe a detailed protocol for the monitoring of neuronal cAMP dynamics during bath application of neurotransmitters and neuromodulators using the genetically encoded cAMP sensor Epac1-camps and scanning laser confocal microscopy.
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