Pyrroline-5-carboxylate

A New Method for Accurately Measuring 1-Pyrroline-5-Carboxylate Synthetase Activity

Proline is a key factor in plant adaptation to environmental stresses. The Δ1 -pyrroline-5-carboxylate synthetase catalyzes the first committed step and the rate-limiting step for proline biosynthesis in both plants and mammals. This enzyme

酵母细胞的异源功能互补

1-pyrroline-5-carboxylate reductase）以及编码脂肪、碳水化合物、核酸和维生素代谢中相关代谢酶的基因。 　　然而，有些真核细胞的酶需要翻译后加工，是不能应用E.coli异源互补法的。为了解决不能对植物基因进行蛋白翻译后加工修饰的问题，人们发展应用了酵母突变体的异源互补法。尽管用于互补的表达文库一般产生融合多肽，有些酶不能忍受任何N-端的延伸，但该法的筛选效率比抗体或杂交高2-3倍，尤其是对那些非常不容易被纯化或不稳定的蛋白（如膜蛋白等）来说，更是如此。应用酵母异源互补法用于分离

EPR Spin-Trapping and Nano LC MS/MS Techniques for DEPMPO/OOH and Immunospin-Trapping with Anti-DMPO Antibody in Mitochondrial Electron Transfer Syste

with 5-diethoxylphosphoryl-5-methyl-1-pyrroline N -oxide (DEPMPO), indicating that FMN/FMN-binding domain (complex I), ubiquinone (complex I and III), FAD/FAD-binding domain (complex II), and cytochrome b (complex III) control the mediation of O2 •– production