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/dsbA重组蛋白DsDNA-binding protein

A(rpbB)Enterobacteria phage T4 Double-stranded DNA-binding protein(dsbA)蛋白
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  • ¥2328
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  • CSB-EP321092EDZ
  • 2026年01月20日
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Enterobacteria phage T4 Double-stranded DNA-binding protein (dsbA)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Enterobacteria phage T4 Double-stranded DNA-binding protein (dsbA)

    品名简称:

    Recombinant Enterobacteria phage T4 dsbA protein

    货号:

    CSB-EP321092EDZ

    规格:

    20μg

    价格:

    2328

    LC-MS/Western Blot:

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP321092EDZ could indicate that this peptide derived from E.coli-expressed

    SDS-PAGE:

    Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP321092EDZ could indicate that this peptide derived from E.coli-expressed Enterobacteria phage T4 (Bacteriophage T4) dsbA.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    dsbA

    Uniprot No.:

    P13320

    别名:

    DsDNA-binding protein A (rpbB)

    种属:

    Enterobacteria phage T4 (Bacteriophage T4)

    蛋白长度:

    Full Length

    来源:

    E.coli

    分子量:

    17.8 kDa

    表达区域:

    1-89aa

    氨基酸序列:

    MAKKEMVEFDEAIHGEDLAKFIKEASDHKLKISGYNELIKDIRIRAKDELGVDGKMFNRLLALYHKDNRDVFEAETEEVVELYDTVFSK

    蛋白标签:

    N-terminal 10xHis-tagged and C-terminal Myc-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    3-7 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    May play a role in transcription of several T4 genes. Binds double-stranded DNA and interacts preferentially with T4 late promoter regions.

    功能2:

    May play a role in transcription of several T4 genes. Binds double-stranded DNA and interacts preferentially with T4 late promoter regions.

    文献:

    "Overexpression and structural characterization of the phage T4 protein DsbA." Sieber P., Lindemann A., Boehm M., Seidel G., Herzing U., van der Heusen P., Muller R., Ruger W., Jaenicke R., Rosch P. Biol. Chem. 379:51-58(1998)

    研究领域:

    Cell Biology

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    相关实验
    • Ligation of DNA with T4 DNA Ligase

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    • Bacteriophage T4 Capsid Packaging and Unpackaging of DNA and Proteins

      Bacteriophage T4 has proven itself readily amenable to phage-based DNA and protein packaging, expression, and display systems due to its physical resiliency and genomic flexibility. As a large dsDNA phage with dispensable internal proteins

    • Purification of DNA from lambda phage

      This is a modification of the procedure in Short Protocols (1-41,1-45) 1.Prepare a 50 ml liquid lysate: A.mix 2xlO8 E.coli cells with 100μl phage (from one picked plaque in 500μl SM),and 100μl lOmM MgCl2/lOmM CaCI2.Adsorb at 37℃ for 15 min. B

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    /dsbA重组蛋白DsDNA-binding protein A(rpbB)Enterobacteria phage T4 Double-stranded DNA-binding protein(dsbA)蛋白
    ¥2328