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- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
- 英文名:
Recombinant Enterobacteria phage T4 (Bacteriophage T4) Recombination and repair protein
- 库存:
1
- 供应商:
北京博蕾德国际贸易有限公司
- 规格:
10ug//20ug//50ug//100ug
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文献和实验Bacteriophage T4 Capsid Packaging and Unpackaging of DNA and Proteins
Bacteriophage T4 has proven itself readily amenable to phage-based DNA and protein packaging, expression, and display systems due to its physical resiliency and genomic flexibility. As a large dsDNA phage with dispensable internal proteins
Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
DNA Ligation and Escherichia coli Transformation
DNA ligase, which requires Mg2+ and NAD+ as cofactors and is less commonly used, and T4 DNA ligase produced by the E. coli phage T4, which requires Mg2+ and ATP as cofactors. The in vivo function of both these enzymes is to repair nicks in double
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