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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human FMN-dependent NADH-azoreductase (azoR)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human FMN-dependent NADH-azoreductase (azoR)品名简称:
Recombinant Human azoR protein货号:
CSB-EP2941HU规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
azoRUniprot No.:
A0A2P9AIQ7别名:
/种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
27.8 kDa表达区域:
1-211aa氨基酸序列:
MSILLVTSSPRGNASHSTRIATELAEKLIAADPSAKLVVRDLVANPLPHIDPDYSTGIYTPVEARTQRQAEVVGVSDGVLDELFAADTIILATGFINFGISSTLKSWVDHVARSGKTFAYGENGPKGLVTGKKVYIVLASGGIYSEGAAVQMDHAIPYLRSVLGFIGMTDVDVIRIEGVGMGPEAVTAALAKASANVDAVVASTGAVAAAA蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
Song W.-J., Kurnit D.M. Submitted (DEC-2016)研究领域:
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文献和实验复合物I又称NADH 脱氢酶(NADH dehydrogenase)或NADH-CoQ 还原酶复合物, 功能是催化一对电子从NADH传递给CoQ,它是线粒体内膜中最大的蛋白复合物,是跨膜蛋白,也是呼吸链中了解最少的复合物。哺乳动物的复合物Ⅰ含有42种不同的亚基,总相对分子质量差不多有1000kDa。其中有7个亚基都是疏水的跨膜蛋白,由线粒体基因编码。复合物Ⅰ含有黄素蛋白(FMN)和至少6个铁硫中心(iron-sulfur centers)。一对电子从复合物Ⅰ传递时伴随着4个质子被传递到膜
黄素蛋白是由一条多肽结合1个辅基组成的酶类,结合的辅基可以是FAD或FMN,它们是维生素B2的衍生物,每个辅基能够接受和提供两个质子和电子。线粒体中的黄素蛋白主要是电子传递链中NADH脱氢酶和TCA循环中的琥珀酸脱氢酶。
结合疏松,当与某酶蛋白结合时可以从代谢物接受氢,而被还原为NADH,后者可以游离,再与另一种酶蛋白结合,释放氢后又被氧化为NAD+。 多数黄素蛋白参与呼吸链组成,与电子转移有关,如NADH脱氢酶(NADh dehydrogenase)以FMN为辅基,是呼吸链的组分之一,介于NADH与其它电子传递体之间;琥珀酸脱氢酶,线粒体内的甘油磷酸脱氢酶(glycerol phosphate dehydrogenase)的辅基为FAD,它们可直接从作用物转移还原当量H++e reducing
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