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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Vaccinia virus Protein A33 (VACWR156), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Vaccinia virus Protein A33 (VACWR156), partial品名简称:
Recombinant Vaccinia virus VACWR156 protein, partial货号:
CSB-YP303065VAI规格:
20μg价格:
2616LC-MS/Western Blot:
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP303065VAI could indicate that this peptide derived from Yeast-expressedSDS-PAGE:
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP303065VAI could indicate that this peptide derived from Yeast-expressed Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) VACWR156.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
VACWR156Uniprot No.:
P68617别名:
VACWR156; A33RProtein A33种属:
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))蛋白长度:
Partial来源:
Yeast分子量:
16.2 kDa表达区域:
57-185aa氨基酸序列:
VRLNQCMSANEAAITDAAVAVAAASSTHRKVASSTTQYDHKESCNGLYYQGSCYILHSDYQLFSDAKANCTAESSTLPNKSDVLITWLIDYVEDTWGSDGNPITKTTSDYQDSDVSQEVRKYFCVKTMN蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Coordinates the incorporation of A36 into wrapped enveloped virion membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.功能2:
Coordinates the incorporation of A36 into wrapped enveloped virion (EV) membranes and, subsequently, the production of actin tails. Therefore plays an essential role in efficient cell-to-cell spread of viral particles.文献:
"Interactions between vaccinia virus IEV membrane proteins and their roles in IEV assembly and actin tail formation." Rottger S., Frischknecht F., Reckmann I., Smith G.L., Way M. J. Virol. 73:2863-2875(1999)研究领域:
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文献和实验Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination
Vaccinia virus, the prototype Orthopoxvirus, is widely used in the laboratory as a model system to study various aspects of viral biology, virus-host interactions, and as a protein expression system and a vaccine vector. The ubiquitous use
Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing
/El, E1/E2, E2/p7, and p7/NS2 sites) (9 ,13 ), whereas an HCV-encoded serine proteinase located in the N-termmal one-third of the NS3 protein is responsible for four cleavages in the NS region (3/4A, 4A/4B, 4B/5A, and 5A/5B sites) (11 ,14 –16 ).
Imaging of Vaccinia Virus Entry into HeLa Cells
. The methods presented here describe how to construct a fluorescent recombinant vaccinia virus expressing the core protein A4L fused to mCherry, and how to detect the virus movement on actin-EYFP-expressed HeLa cells.
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