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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Uncharacterized protein YncE (yncE)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Uncharacterized protein YncE (yncE)品名简称:
Recombinant E.coli Uncharacterized protein YncE protein货号:
CSB-EP302812ENV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
yncEUniprot No.:
P76116别名:
/种属:
Escherichia coli (strain K12)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
36.3 kDa表达区域:
31-353aa氨基酸序列:
AEEMLRKAVGKGAYEMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQTLWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGIGKESVIWVVDGGNIKLKTAIQNTGKMSTGLALDSEGKRLYTTNADGELITIDTADNKILSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLAVLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQKSTKQQEATQPDDVIRIAL蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Preliminary crystallization indicates the presence of a single beta-propeller domain that contains 7 4-stranded beta-sheets.功能2:
/文献:
"Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli." Baba-Dikwa A., Thompson D., Spencer N.J., Andrews S.C., Watson K.A. Acta Crystallogr. F 64:966-969(2008)研究领域:
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文献和实验先来看下传统重组蛋白表达系统: 传统重组蛋白表达系统应对的挑战: 操作流程长:5天-4周时间 很多蛋白不能在细胞内成功表达 不可以改造目标蛋白 以大肠杆菌为代表的原核蛋白表达系统是常用的表达系统,具有表达量高和成本低的优点,但是蛋白质翻译后缺乏加工机制(比如形成二硫键和蛋白糖基化等),且很难保留生物活性。 以甲醇毕赤酵母为代表的酵母蛋白表达系统,具有表达量高,可诱导,糖基化机制接近真核生物等优点,但是部分蛋白产品易降解,表达量不可控。 哺乳动物细胞和昆虫
Loukachevitch, L.V. and Egli, M. 2007. Crystallization and preliminary X‐ray analysis of Escherichia coli RNase HI‐dsRNA complexes. Acta Cryst. F 63:84‐88. Sam
表达容易形成不溶的聚集体,称为“包涵体”。大肠杆菌表达产生包涵体有以下优点[1,2]: 1) 以包涵体形式表达的蛋白质的浓度比较高,有时甚至可以达到细胞总蛋白含量的30% [3]; 2) 如果重组蛋白质以包涵体形式存在,由于包埋了酶攻击的位点,可以最大限度地抵抗蛋白质酶的攻击; 3) 包涵体表达的蛋白质没有活性,细胞破碎和以后的纯化步骤,不用考虑蛋白质的失活问题; 4) 包涵体形式表达的蛋白质与宿主细胞的其他蛋白质的分离比可溶蛋白质的分离方法简便,造价低,使用简单的离心或者过滤
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