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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bovine Allograft inflammatory factor 1 (AIF1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bovine Allograft inflammatory factor 1 (AIF1)品名简称:
Recombinant Bovine AIF1 protein货号:
CSB-EP001490BO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
AIF1Uniprot No.:
Q9BDK2别名:
/种属:
Bos taurus (Bovine)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
27.6 kDa表达区域:
2-147aa氨基酸序列:
SETRDLQGGKAFGLRKAQQEERINEINQQFLDDPKYSSDEDLPSKLEAFKKKYMEFDLNEDGGIDIMSLKRMMEKLGVPKTHLELKKLIMEVSSGPGETFSYSDFLKMMLGKRSAILKMILMYEEKAREQEKPTGLPAKKAISELP蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May play a role in macrophage activation and function.功能2:
/文献:
"Increased messenger RNA for allograft inflammatory factor-1, LERK-5, and a novel gene in 17.5-day relative to 15.5-day bovine embryos." Glover M.D., Seidel G.E. Jr. Biol. Reprod. 69:1002-1012(2003)研究领域:
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文献和实验to treat immunopathology, these cells act in a very specific manner. Consequently, their clinical potential in the treatment of autoimmune disorders, inflammatory diseases, graft-versus-host disease, and allograft rejection is currently extensively studied
Immunity:厦门大学韩家淮团队揭示 PELO 控制 NOD 样受体家族蛋白寡聚化组装和激活的机制
的炎症小体(Inflammasome),NOD2 等形成的 Nodosome,从而激活 NF-κB 通路、MAPK 通路、细胞焦亡等,释放 TNFα,IL-1β 和 IL-18 等炎性细胞因子,介导下游一系列免疫炎症级联反应【1,2】。这是机体最为基本的天然免疫防御反应之一,在机体清除病原感染和内源危险信号中发挥至关重要的作用。在人体中,多个 NLR 基因突变导致的异常激活也被发现参与了脓毒症、炎性肠病等多种重大炎性疾病的病理进程。 NLR 家族蛋白在结构上都含有一个核苷酸结合寡聚化结构域
浅谈流式核内蛋白染色步骤: 1、染表面抗体 按说明书条件孵育全血样品孵育抗体后加入不含固定剂的溶血素(按厂家说明操作)溶血完毕。 2、用2ml预冷流式染色缓冲液(Flow Cytometry Staining Buffer)洗涤细胞300-400g离心5分钟,去上清。 3、用3倍体积的固定/破膜稀释液(#00-5223)稀释固定/破膜浓缩液(#00-5123)制成固定/破膜工作液,注意要新鲜配制,每个样本的用量为1ml 。 4、旋涡震荡重悬细胞后加入1ml的固定/破膜工作
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