- ￥260000 - 310000
- Thermo Fisher
- A28567
- 新家坡
- 2026年01月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
9台
- 保修期：
1Y
- 现货状态：
3台
- 供应商：
广州维基科技有限公司
- 规格：
套
The Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an
affordable, easy-to-use real-time PCR system that doesn't compromise performance and quality.
The simplified Design and Analysis software is ideal for both first-time and experienced users.
When connected to iConnect, Thermo Fisher's cloud-based platform, the QuantStudio 3 system
provides access to your data wherever and whenever you want. Using proven OptiFlex technology
(featuring four coupled channels and white LED) and featuring three independent Veriflex temperature
zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of
genomic applications.
Quantstudio 3/5 Firmware and Design & Analysis Software >
Features of the QuantStudio 3 system include:
• Simplified instrument software with interactive touchscreen for greater ease of use
• Proven OptiFlex technology (four coupled channels with white LED) for trusted results
• Secure cloud-based software option provides increased accessibility and storage options
• User account management, locked workflow, and Pause features for greater control of experiments
• 96-well 0.2 mL format or 96-well 0.1 mL format
iConnect
iConnect provides scientists with a secure place to store, analyze, and share data. Upload data from
the instrument or upload your own files from anywhere you have web access. Applied Biosystems
qPCR Analysis Modules within iConnect are web browser-based software tools that are flexible,
fast and easy to use.
Easy to use
The QuantStudio 3 Real-Time PCR System is compact in form, factory calibrated and is
delivered in user-friendly packaging to enable fast and simple installation for immediate use.
The interactive touchscreen provides a modern usability experience and the simplified
instrument software/pre-optimized protocol templates allow users of any experience level to
quickly set up and start a run.
Design and analysis software options:
Option 1: QuantStudio Design and Analysis cloud software
• Supports web browser-based system configuration with PC or MAC computer
• Streamlined software for improved usability and response time
• Enables secure access of your data when and where you want through the cloud
• No software to install, no licenses and no versions to update
Option 2: QuantStudio Design and Analysis desktop software
• Supports traditional co-located computer system configuration on Windows 10 OS
• Streamlined software for improved usability and response time
• No licenses to purchase
Results you can trust
Improved well-to-well and instrument-to-instrument data accuracy is achieved with
OptiFlex technology, which features four coupled excitation and emission filter channels with white LED.
Sensitive data analysis
Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.
High application versatility
Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for
a broad range of applications, including gene expression, genetic variation, gene regulation, and more.
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文献和实验光信号的收集，这是我们荧光定量 PCR 反应的关键。而同样的我们需要在哪一步来收集信息呢？其实我们只需要在扩增循环中的退火或延伸步骤采集荧光即可（图 8）。 图 8 退火/延伸步骤采集荧光信号 不正确的信号采集同样会造成扩增曲线的异常。如下图所示，客户使用 QuantStudio™ 6 仪器开展实验，实验结束后发现扩增曲线不显示且多组分图表现异常（图 9A，9B），更进一步排查其反应程序设置可以发现，在扩增循环阶段除了在退火/延伸步骤收集荧光外，95℃ 变性步骤的荧光采集开关也被打开了（图 9C
Biosystems™ 系列荧光定量 PCR 仪加热模块分 0.2 ml 跟 0.1 ml 两种，实验前一定要确定自己的仪器是哪一种加热模块，再来选择对应的耗材。如果 0.1 ml 加热模块用成 0.2 ml 耗材，则会造成耗材压扁，甚至造成机器故障；如果 0.2 ml 加热模块用成 0.1 ml 耗材，则会造成反应管的外壁和荧光定量 PCR 仪器的温控模块没有充分接触，从而出现热传导不充分，进而影响酶的活性，会引起重复性不好（图四），或者溶解曲线多峰（非特异扩增），甚至是假阴性结果； 图四：耗材规格跟加热
的 microRNA； ● 超宽的线性范围，可跨越 7 个数量级。 实时定量 PCR 的系统构成及工作原理 荧光定量检测系统由实时荧光定量 PCR 仪，实时荧光定量试剂，通用电脑，自动分析软件等构成。设备由荧光定量系统和计算机组成，用来监测循环过程的荧光。 与实时设备相连的计算机收集荧光数据。数据通过开发的实时分析软件以图表的形式显示。原始数据被绘制成荧光强度相对于循环数的图表。原始数据收集后可以开始分析。实时设备的软件能使收集到的数据进行正常化处理来弥补背景荧光的差异。正常化后可以设定域值水平
