相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
SARS-CoV-2 (2019-nCoV) Spike RBD (K417T) Protein (rFc Tag)
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation K417T) (Arg319-Phe541) was expressed with the Fc region of rabbit IgG1 at the C-terminus.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Ser
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 448 amino acids and predicts a molecular mass of 50.4 kDa. As a result of glycosylation, it migrates as an approximately 56.5 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验特异性结合的单克隆抗体1。与Rho1D4抗体结合的表位可以作为针对膜蛋白的超高特异性纯化标记。通过基因修饰可在欲研究的目标(膜)蛋白C端加上Rho1D4标记。一旦加载上该序列,即可用装载有Rho1D4抗体的亲和基质去捕获目标蛋白,随后添加Rho1D4肽,通过竞争性结合基质抗体的方式来洗脱目标蛋白。采用这种方法,相比改变pH值等其他洗脱方式,更加地温和。图1:假设有3个跨膜域的膜蛋白。Rho1D4标签加到膜蛋白C端,它的序列是T-E-T-S-Q-V-A-P-A2) Rho1D4的历史Rho1D4的表位
日前,北京大学生命科学学院魏文胜课题组在 Cell 杂志上在线发表题为“Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants”的研究论文。 魏文胜团队首先建立了体外高效制备高纯度环状 RNA 的技术平台,针对新型冠状病毒及其变异株,设计了编码新冠病毒刺突蛋白(Spike)受体结构域(RBD)的环状 RNA 疫苗。 该项研究中制备的针对新冠病毒德尔塔变异株的环状 RNA 疫苗(circRNARBD
&Tag 操作流程[5] CUT&Tag 能够对极少量细胞( 60 个细胞)进行操作,还可以进行单细胞操作。进行单细胞操作需要将细胞进行分装,由于 Protein A-Tn5 在细胞内进行,PCR 反应之前,切割的核酸均在细胞内, Henikoff 博士通过分配单个细胞到 5184 纳米孔,再加入带随机标签的引物进行扩增的办法,实现单细胞测序。 ✦其他蛋白质-DNA 互作研究方法: 2019 年 3 月以来,bioRxiv 上传了数篇蛋白-DNA 互作研究的预印文章,包括
技术资料暂无技术资料 索取技术资料









