义翘神州 N501Y-新冠突变重组蛋白 Fc标签 HEK293表达

SARS-CoV-2 (2019-nCoV) Spike RBD (N501Y) Protein (Fc Tag): 产品信息
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind S3-2019-nCov-2-RBD(319R-541F,N501Y)-Fc(Cat:40592-V02H1), the EC50 of S3-2019-nCov-2-RBD(319R-541F,N501Y)-Fc(Cat:40592-V02H1) is 1.5-8.0 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation N501Y) (Arg319-Phe541) was expressed with the Fc region of human IgG1 at the C-terminus. The mutation were identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Ser
分子量The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 462 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, it migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.