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Coronavirus spike重组蛋白|Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein
- ￥4500
- Sino Biological
- 北京
- 40592-V08H82
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
新冠病毒SARS-CoV-2 Spike RBD(N501Y)-His 重组蛋白（产品说明）
蛋白名称：新冠病毒N501Y突变体蛋白
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(N501Y)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His(40592-V08H82), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His (40592-V08H82) is 3-20 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541(N501Y)
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：新冠病毒N501Y突变体蛋白
蛋白构建：A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein (YP_009724390.1) (Arg319-Phe541(N501Y)) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His(40592-V08H82), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His (40592-V08H82) is 3-20 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 319
蛋白分子量：The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein consists of 234 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 35 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：YP_009724390.1
蛋白氨基酸序列：Arg319-Phe541(N501Y)
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Makdasi E, et al. The neutralization potency of anti-SARS-CoV-2 therapeutic human monoclonal antibodies is retained against viral variants.Cell reports, PubMed ID: 34464610
2, Tang J, et al. Epitope diversity of SARS-CoV-2 hyperimmune intravenous human immunoglobulins and neutralization of variants of concern.iScience, PubMed ID: 34430803
3, Tang J, et al. Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sex.EBioMedicine, PubMed ID: 34902788
4, Konrath KM, et al. Nucleic acid delivery of immune-focused SARS-CoV-2 nanoparticles drives rapid and potent immunogenicity capable of single-dose protection.Cell reports, PubMed ID: 35090597
5, Lee Y, et al. SARS-CoV-2 mRNA vaccine induced higher antibody affinity and IgG titers against variants of concern in post-partum vs non-post-partum women.EBioMedicine, PubMed ID: 35301181
6, Shim JE, et al. Single-Nanoparticle-Based Digital SERS Sensing Platform for the Accurate Quantitative Detection of SARS-CoV-2.ACS applied materials & interfaces, PubMed ID: 35951983
7, Kim JW, et al. Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2.Biomedicines, PubMed ID: 36552031
8, Tai W, et al. An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants.Nature communications, PubMed ID: 37221158
9, Rozak H, et al. Adsorption of SARS-CoV-2 Spike (N501Y) RBD to Human Angiotensin-Converting Enzyme 2 at a Lipid/Water Interface.The journal of physical chemistry. B, PubMed ID: 37171105
10, Park S, et al. An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutation.Nature communications, PubMed ID: 38643233
2, Tang J, et al. Epitope diversity of SARS-CoV-2 hyperimmune intravenous human immunoglobulins and neutralization of variants of concern.iScience, PubMed ID: 34430803
3, Tang J, et al. Antibody affinity maturation and cross-variant activity following SARS-CoV-2 mRNA vaccination: Impact of prior exposure and sex.EBioMedicine, PubMed ID: 34902788
4, Konrath KM, et al. Nucleic acid delivery of immune-focused SARS-CoV-2 nanoparticles drives rapid and potent immunogenicity capable of single-dose protection.Cell reports, PubMed ID: 35090597
5, Lee Y, et al. SARS-CoV-2 mRNA vaccine induced higher antibody affinity and IgG titers against variants of concern in post-partum vs non-post-partum women.EBioMedicine, PubMed ID: 35301181
6, Shim JE, et al. Single-Nanoparticle-Based Digital SERS Sensing Platform for the Accurate Quantitative Detection of SARS-CoV-2.ACS applied materials & interfaces, PubMed ID: 35951983
7, Kim JW, et al. Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2.Biomedicines, PubMed ID: 36552031
8, Tai W, et al. An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants.Nature communications, PubMed ID: 37221158
9, Rozak H, et al. Adsorption of SARS-CoV-2 Spike (N501Y) RBD to Human Angiotensin-Converting Enzyme 2 at a Lipid/Water Interface.The journal of physical chemistry. B, PubMed ID: 37171105
10, Park S, et al. An ancestral SARS-CoV-2 vaccine induces anti-Omicron variants antibodies by hypermutation.Nature communications, PubMed ID: 38643233
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Coronavirus spike重组蛋白|Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein
￥4500
