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- Sigma-Aldrich
- 进口
- P7949-500ML
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
TWEEN ® 20
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
9005-64-5
- 规格：
500ML
属性
生物来源
plant
质量水平
300
描述
non-ionic
产品线
BioXtra
形式
viscous liquid
分子量
~1228
组成
lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)
技术
blocking: suitable
cell culture | mammalian: suitable
hybridization: suitable
protein purification: suitable
transformation: suitable
杂质
≤0.05% Phosphorus (P)
灼烧残渣
≤0.2%
折射率
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
转变温度
cloud point 76 °C
密度
1.095 g/mL at 25 °C (lit.)
痕量阴离子
chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%
痕量阳离子
Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%
HLB
16.7
SMILES字符串
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
一般描述
表面活性剂的特征在于同时具有亲水和疏水成分，根据其在水中的电离行为可以分为离子型（阴离子、阳离子或两性）或非离子型 。所述产品属于非离子型，可用于各种应用，包括乳化、分散、增溶和去污剂。此外，它也可用作膜蛋白的增溶剂。
应用
吐温20在基于膜的免疫分析中已被用作一种封闭剂，其常用浓度为0.05%。吐温20可用于裂解哺乳动物细胞，使用浓度为0.005至0.5%。
特点和优势
- 用于生命科学和生物化学研究的多功能表面活性剂
- 用于灵敏应用的BioXtra级产品
- 测试过痕量阴离子和阳离子
- 良好的增溶、分散和乳化剂
- 适用于哺乳动物细胞培养、蛋白质纯化和杂合研究
注意
制备说明
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文献和实验TFE3-PD-L1 axis is pivotal for sunitinib resistance in clear cell renal cell carcinoma.
The microphthalmia of bHLH-LZ transcription factor (MiT/TFE) family chromosomal translocation or overexpression is linked with a poor prognosis in clear cell renal cell carcinoma (ccRCC) with elevated recurrence and drug resistance, but the molecular mechanism is not fully understood. Here, we investigated whether the resistance to sunitinib (Sun), the standard treatment for metastatic ccRCC, is due to up-regulation of programmed death ligand 1 (PD-L1) by the transcription factor E3 (TFE3). In this study, we propose that TFE3 but not TFEB is essential for tumour survival which was associated with the poorer survival of cancer patients. We also found a positive correlation between TFE3 and PD-L1 expression in ccRCC cells and tissues. Sun treatment led to enhanced TFE3 nuclear translocation and PD-L1 expression. Finally, we observed the therapeutic benefit of Sun plus PD-L1 inhibition which enhanced CD8+ cytolytic activity and thus tumour suppression in a xenografted mouse model. These data revealed that TFE3 is a potent tumour promoting gene and it mediates resistance to Sun by induction of PD-L1 in ccRCC. Our data provide a strong rationale to apply Sun and PD-L1 inhibition jointly as a novel immunotherapeutic approach for ccRCC treatment.
licanming 请问大家用sigma公司的PMA时，储存液的浓度是配多少的？保存期多长？我是准备用DMSO配，我看说明书的意思是储存液浓度为20mM，但我工作液的浓度只要100nM，故很难分装和稀释。如果储存液浓度低一点，会不会对保存时间产生影响啊？我在园子里搜过，有用1mg/ml的，但我不知他是否用sigma公司的。 licanming 自己顶一下 amygdala 对于保存
E.Z.N.A.® Mag-Bind® Viral DNARNA Spin Protocol
at room temperature for 10 min. Note: Add 20 ul 2-mercaptoethanol per 1 ml of QVL Lysis Buffer before use. This mixture can be made and stored at room temperature for 1 week. 3. Add 500ul Isopropanol followed with mix thoroughly by vortexing
E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol
Glycogen and 20ul Mag-Bind® Magnetic Beads R to the sample. Vortex at maxi speed for 30 seconds. Incubate at room temperature for 10 min. Note: Add 20 ul 2-mercaptoethanol per 1 ml of QVL Lysis Buffer before use. This mixture can be made and stored
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