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文献和实验Quenched Fluorescent Substrate- Based Peptidase Assays
The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to highthroughput screening of potential peptidase inhibitors. In this chapter, we discuss
DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
: TBS 250 μl/well; 3x 30 seconds.8. Amplify signal by adding 100 μl/well AttoPhos Fluorescent substrate system, for 5-10 min at RT. 36 mg ofAttoPhos substrate should be mixed with 60 ml of AttoPhos buffer 24 hours prior to use. Make sure well isclean
Lipolytic enzymes have traditionally been assayed by radiometric and titrimetric methods (1 ). Radiometric methods are quite sensitive but require expensive radiolabeled substrates and tedious separation of labeled substrate and products
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