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- Roche
- 11969056001
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海益启生物科技有限公司
- 规格：
1250 μL
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文献和实验with mineral oil or paraffin (melting temperature 50-60°C) of special PCR grade. One-half of the total reaction volume is usually sufficient. Temperature Cycling Amplification parameters depend greatly on the template, primers and amplification apparatus
Freeze Squeeze PCR Product Purification from Agorose Gel
Abstract: The “Freeze squeeze” method is a cost effective and technically simple procedure to isolate and prepare PCR products for DNA sequencing. Reagents •70% ethanol •3 M sodium acetate Procedure 1.Run your PCR product of interest on a 1
Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA
with Uracil-DNA Glycosylase (UNG) prior to the thermal cycling in combination with the use of dUTP in the PCR amplification is a commonly used technology to prevent such cross-contamination. Since sodium bisulfite treatment converts unmethylated cytosine bases
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