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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
Influenza A H3N2 (A/Hong Kong/2671/2019) Nucleoprotein / NP Protein (His Tag)
- 保质期:
12个月
- 供应商:
北京义翘神州科技股份有限公司
- 保存条件:
-20℃ to -80℃
- 规格:
100 µg
纯度> 95 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白构建A DNA sequence encoding the Influenza A virus (A/Hong Kong/2671/2019 (H3N2)) nucleoprotein (translated amino acids of EPI1698482) (Met1-Asn498) was expressed with a polyhistidine tag at the C-terminus.
EP No.EPI1698482
表达宿主Baculovirus-Insect Cells
种属H3N2
预测 N 端Met 1
分子量The recombinant nucleoprotein of Influenza A virus (A/Hong Kong/2671/2019 (H3N2)) consists of 509 amino acids and predicts a molecular mass of 57.3 kDa.
缓冲液Lyophilized from sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验核蛋白提取 实验准备: 1 4℃离心机 2 根据标本数计算所需I,II,III,IV总体积,取出液体,并加入蛋白酶抑制剂,(III由加蛋白酶抑制剂I与加蛋白酶抑制剂II混合物) 实验操作: 1 收集细胞。10ml 对照细胞(1-2*105/ml )培养48h后,15ml 离心管收集,300g(1370 rpm)* 5min。弃上清,加1ml 1*PBS轻轻涡旋混匀后转移至1.5ml EP管。 2 4℃,300g * 5min,弃上清。 3 重悬
质组学。 我们这里描述的这种方法适用于未知基因序列的植物。集中研究少数蛋白质,这些蛋白质存在于已知功能的不同馏分中。因此,我们研究亚细胞结构的蛋白质组学,即细胞核研究,并且将它分为亚蛋白质组进行研究。 很明显,第一步是获取生物材料以用来研究。因为我们感兴趣于研究核蛋白相关的细胞增殖,所以我们选择根尖分生组织细胞群。材料是匀浆后纯化细胞器(此处为细胞核)。从分生组织细胞中分离细胞核比较容易,因为这类细胞核占据很大体积。 从这些被纯化的细胞核中,通过逐渐增加缓冲液离子强度而逐渐增大蛋白溶解度,依次得到的蛋白
目录 美籍日裔科学家川冈(Kawaoka)报告,含有1918年病毒RNA聚合酶复合物基因(PA、PB1和PB2)和核蛋白基因(NP)是当年病毒致命的原因。相关论文日前发表于《美国科学院院刊》[Proc Natl Acad Sci U S A 2008, 106(2):587]。 研究者使1918病毒和H1N1病毒株基因重排(reassortant),随后在动物试验中寻找毒力最强的基因组合。他们发现,大多数的重排组合病毒株,毒力与一般的流感病毒类似。在雪貂
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