Acetyl-α-Tubulin (Lys40) (6-11

Purification of mAb (IgG)

1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH

Purification of mAb (IgG)

  Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos

, add the transcription reaction onto them and spin 15 min. The volume of the final eluate should be around 30-40µl . Run a gel (loading 1.5µl in 5µl loading buffer) to quantify the yield and to determine the amount to be used