- ￥6300
- GeneTex
- 美国
- GTX16292
- 2025年07月15日
- WB, ICC/IF, IHC-Wm, Dot, ELISA, EM, RIA
- Mouse
- Human, Mouse, Rat, Zebrafish, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Frog
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Frog
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Sea Urchin
- 目录编号：
GTX16292
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-Wm, Dot, ELISA, EM, RIA
- 浓度：
Batch dependent(Please refer to the vial label for the specific concentration.)
- 靶点：
Tubulin (Acetyl Lys40)
- 抗体英文名：
Tubulin (Acetyl Lys40) antibody [6-11B-1]
- 抗体名：
Tubulin (Acetyl Lys40) 抗体 [6-11B-1]
- 规格：
100 μl
WB analysis of (1)HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cells using GTX16292 Tubulin (Acetyl Lys40) antibody [6-11B-1] at 1:2,000.
ICC/IF analysis of HeLa cells using GTX16292 Tubulin (Acetyl Lys40) antibody [6-11B-1] at 1:2000 (red) with DAPI(blue). Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton™ X-100.
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文献和实验Huang PY et al., Am J Cancer Res 2022 (PMID:35261809)
Nelson HM et al., Dev Dyn 2019 (PMID:31310039)
Sanchez E et al., Mol Neurobiol 2018 (PMID:29344929)
Yang H et al., Cytoskeleton (Hoboken) 2011 (PMID:21309084)
Wen-Jui Lee et al., Molecules 2021 (PMID:34770956)
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
Immunofluorescent Localization of Tubulin
and rinse 2X with PBS. Prepare a 1/40 anti-tubulin dilution using PBS. PbS alone may be used or better, augment the PBS with 3% (w/v) Bovine Serum Albumin (BSA). It may be necessary to check the appropriate antibody dilution
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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