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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
followed by elution with desthiobiotin. (2) Purification by the FLAG‐tag moiety: binding to anti‐FLAG M2 affinity matrix followed by elution with FLAG peptide. Abbreviations: sp., specific interactors (shown as gray circles); n.sp., nonspecific proteins
on Affinity Chromatography, Principles and Methods (18-1022-29), which can be downloaded from www.gehealthcare.com/protein-purification3. Using an adaptor Adaptors should be fitted as follows: 1. After the medium has been packed as described
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