- ￥4300
- GeneTex
- 美国
- GTX26673
- 2025年07月07日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC
- Goat
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- 文献和实验
- 技术资料
- 免疫原：
Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX26673
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Goat
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC
- 浓度：
1.03 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Anti-GFP assayed by ELISA for direct binding of antigen recognizes wild type, recombinant and enhanced forms of GFP
- 抗体英文名：
GFP antibody
- 抗体名：
GFP 抗体
- 规格：
100 μg
Figure 1. Western blot of GFP recombinant protein detected with GeneTex polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kDa band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. After blocking the membrane was probed with the primary antibody diluted to 1 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of donkey anti-Goat IgG [H&L].
Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification). GeneTex polyclonal goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect eGFP. Fluorchrome conjugated anti-goat IgG secondary antibody was used for detection at 1:500. Sections were counterstained with DAPI. Personal Communication, Daniel A. Lee, Johns Hopkins School of Medicine, Baltimore, MD.
Figure 2. Immunofluorescence microscopy. GeneTex polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GFP in cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) in Drosophila melanogaster late stage embryonic central nervous system. Fluorochrome conjugated anti-Goat secondary antibody was used for detection at 1:300. Panel A shows a lateral view (ventral left) and Panels B and C show ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). Personal Communication, Helmata Mistry, Washington University School of Medicine, St. Louis, MO.
Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.
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文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
对照，2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R]（货号#E022030），其免疫原是KLH偶联的水母GFP蛋白的N末端多肽，由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致，因此从免疫原上来说，GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是，EarthOx对于该单克隆抗体还进行了明确的验证试验，试验也表明该Anti-GFP Tag Monoclonal
dingxiangyua 有哪位高手知道F-GFP和GFP-actin的抗性分别是什么?急求 Fasta921 定位在膜上的GFP （F-GFP） 绿荧光蛋白(GFP)标记肌动蛋白(GFP-Actin) 抗性？不明白！ biyuntian478 lz是啥意思？ 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验
naj2007 请教一下各位老师，在构建过表达载体的时候，VEGF和GFP是不是不可以做成融合蛋白，但是如果用不同的启动子的话，很容易丢失荧光，有没有什么解决方法。 zhujoker 可以做成融合蛋白的，即使是使用不同的启动子的话荧光也不那么容易丢失的，大不了你将荧光的启动子换成强的如CMV，EF1a之类。 kinguangjun 或者你可以用CMV-VEGF-IRES-GFP来做
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