- 询价
- Tanda
- TD-AB0035
- 2025年12月15日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 抗体英文名：
epidermal growth factor receptor
- 抗体名：
ERBB; HER1; mENA; ERBB1
- 规格：
25 μl/100 μl/200 μl
EGFR mouse monoclonal antibody
| Product Name | EGFR mouse monoclonal antibody |
| Cat.# | TD-AB0035 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | Anti-EGFR mouse monoclonal antibody |
| Applications | WB |
| Immunogen | Fusion protein of EGFR |
| Reactivity | Human |
| Host species | Mouse |
| Ig class | IgG1 |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | EGFR |
| Full name | epidermal growth factor receptor |
| Synonyms | ERBB; HER1; mENA; ERBB1 |
| Target Background | |
| The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily.This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer | |
| Western blotting | |
| Predicted band size | 134 kDa |
| Positive control | SKOV3 and PC3 cell lysates |
| Recommended dilution | 1000-5000 |
| Gel | 6%SDS-PAGE |
| Lysate | 40 μg |
| Lane 1-2 | SKOV3 and PC3 cell lysates |
| Primary antibody | D191065(EGFR Antibody) at dilution 1/600 |
| Secondary antibody | Goat anti mouse IgG at 1/5000 dilution |
| Exposure time | 3 minutes |
| Product Image | |
 | |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
技术资料暂无技术资料 索取技术资料
