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- Tanda
- TD-AB0023
- 2025年12月14日
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- 详细信息
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- 技术资料
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 抗体英文名：
Anti-ERK1/2 Monoclonal A
- 规格：
25 μl/100 μl/200 μl
ERK1/2 mouse monoclonal antibody
| Product Name | ERK1/2 mouse monoclonal antibody |
| Cat.# | TD-AB0023 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | Anti-ERK1/2 Monoclonal A |
| Applications | WB |
| Immunogen | A synthetic peptide coupled |
| Reactivity | Human Mouse Rat |
| Host species | Mouse |
| Ig class | IgG1 |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | |
| Synonyms | ERK; ERT1; MAPK1/2 |
| Target Background | |
| ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine /threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1. | |
| Western blotting | |
| Predicted band size | 44/42kDa |
| Positive control | Recombinant human ERK1 protein |
| Recommended dilution | 1:1,000-2,000 |
| Product Image | |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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