- 询价
- Tanda
- TD-AB0016
- 中国
- 2025年12月17日
- WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Beta tubulin mouse monoclonal antibody
- 抗体英文名：
Beta tubulin mouse monoclonal antibody
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
1年
- 抗原来源：
Mouse
- 级别：
科研级
- 库存：
800
- 供应商：
武汉天德
- 克隆性：
单克隆
- 保存条件：
PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
- 形态：
液体
- 亚型：
gG3
- 免疫原：
Fusion protein of human TUBB
- 规格：
100μl
Beta tubulin mouse monoclonal antibody
Cat.# TD-AB0016
Package 25 μl/100 μl/200 μl
Storage PBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description Anti-Beta tubulin mouse monoclonal antibody
Applications WB
Immunogen Fusion protein of human TUBB
Reactivity Human, Mouse, Rat
Host species Mouse
Ig class IgG3
Purity Protein A/G Affinity Purification
Target information
Symbol beta Tubulin
Full name Tubulin beta chain
Target Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
Western blotting
Predicted band size 50 kDa
Positive control: Mouse brain tissue, Rat brain tissue, Human cerebrum tissue, Human cerebella
tissue lysates
Recommended dilution: 1000-5000
Gel: 8% SDS-PAGE
Lysate: 40 μg
Lane 1-4: Mouse brain tissue, Rat brain tissue,Human cerebrum tissue, Human cerebella tissue
lysates
Primary antibody: D191046(TUBB Antibody) at dilution 1/1200
Secondary antibody: Goat anti mouse IgG at 1/5000 dilution
Exposure time: 3 minutes
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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