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- 2025年10月06日
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Shanghai Model Organisms Center, Inc.
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文献和实验Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E . coli system for test expressing intracellular
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
Phage-Display Libraries of Murine and Human Antibody Fab Fragments
randomly paired light (L) and heavy (H) chain-coding regions under transcriptional control of P lac . The L (or H) chain-coding region is fused in-frame with the truncated phage gene, ΔgIII , coding for a truncated version of the phage surface protein pill
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