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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant full length protein of active H-Ras
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白表达
- 目录编号:
TD-26909
- 级别:
科研级
- 库存:
88
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
- 浓度:
1mg/ml
- 靶点:
Ras
- 抗体英文名:
Anti-Ras-GTP Mouse Monoclonal Antibody
- 抗体名:
Ras-GTP
- 规格:
30 μL
Ras-GTP
| Cat. #: TD-26909 |
| Size: 30 µL |
| Gene Symbol: RAS |
| Description: Anti-Ras-GTP Mouse Monoclonal Antibody |
| Background: Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| Immunogen: Recombinant full length protein of active H-Ras |
| Applications: IP, IHC and IF (Not applicable for WB since WB denatures the GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Ras-GTP monoclonal antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
|
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文献和实验, which are active in the GTP-bound form and inactive in the GDP-bound form. Given the importance of studies on the Ras protein activation state to understand the detailed mechanism of Ras-mediated signal transduction, we provide here a simple
Ras是大鼠肉瘤(rat sarcoma,Ras)的英文缩写。Ras蛋白是原癌基因 c—ras的表达产物,相对分子质量为21kDa,属单体 GTP结合蛋白,具有弱的 GTP酶活性。Ras蛋白的活性状态对细胞的生长、分化、细胞骨架、蛋白质运输和分泌等都具有影响,其活性则是通过与GTP或GDP的结合进行调节。 Ras的活性受两个蛋白的控制,一个是鸟苷交换因子(guanine nucleotide exchange factor, GEF),它的作用是促使GDP从Ras蛋白
小GTP结合蛋白(small GTP binding protein)
), 它催化GDT同GTP的交换。另一种是GTP 酶激活蛋白(GTPase-activating protein, GAP), 它触发结合的GTP水解。 许多小GTP结合蛋白都有一个共价结合的脂基团, 帮助它们同膜结合。小GTP蛋白包括ras、rab和rho蛋白, 它们在一些重要的的活动中起调节作用, 包括分泌活动和细胞骨架的装配等。
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