Ras-GTP

Ras-GTP

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  • ¥4800
  • Bioyears
  • TD-26909
  • 美国
  • 2025年12月10日
  • IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
  • Mouse
  • 脊椎动物
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    • 详细信息
    • 技术资料
    • 供应商

      Bioyears

    • 库存

      88

    • 靶点

      Ras

    • 级别

      科研级

    • 目录编号

      TD-26909

    • 克隆性

      单克隆

    • 抗原来源

      蛋白表达

    • 保质期

      24个月

    • 抗体英文名

      Anti-Ras-GTP Mouse Monoclonal Antibody

    • 抗体名

      Ras-GTP

    • 标记物

      FITC

    • 宿主

      Mouse

    • 适应物种

      脊椎动物

    • 免疫原

      Recombinant full length protein of active H-Ras

    • 亚型

      IgG

    • 形态

      液体

    • 应用范围

      IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)

    • 保存条件

      -20℃

    • 浓度

      1mg/ml

    • 规格

      30μl

    Ras-GTP

    Cat. #:   TD-26909
    Size:  30 µL
    Gene Symbol:  RAS
    Description:   Anti-Ras-GTP Mouse Monoclonal Antibody
    Background:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen:   Recombinant full length protein of active H-Ras
    Applications:   IP, IHC and IF (Not applicable for WB since WB denatures the GTPase)
    Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
    Concentration:   1 mg/ml
    Host Species:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Preservative:  No
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti-Ras-GTP monoclonal antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
    Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing

    Immunoprecipitation/Western blot:  

    Ras-GTP
    IP/WB Analysis of Active Ras Protein.Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Ras-GTP monoclonal antibody (Cat. # TD-26909). After SDS/PAGE, the membrane filter was probed with anti-Ras mouse monoclonal antibody .

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