- ￥7800
- Tanda
- USA
- TD-82901
- 2025年11月08日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Arl1 Pull-Down Activation Assay Kit
- 保质期：
24个月
- 保存条件：
store at -20℃
- 库存：
8000
- 供应商：
武汉天德
- 规格：
30 assays
Arl1 Pull-Down Activation Assay Kit
Cat. # TD-82901
Background
Arf-like protein 1 (Arl1) is a member of the Arf family of regulatory GTPases, within the Ras superfamily of GTPases, and with highly conserved orthologs throughout eukaryotes. Arl1 is essential for early embryonic development in Drosophila and in Caenorhabditis elegans. Arl1 is most similar in primary sequence, cellular location, and function (regulation of membrane traffic) to Arf1–6 and even shares several common binding partners. In addition to its function in membrane traffic at the Golgi/trans-Golgi network, there are reports indicating a possible role for Arl1 in ion homeostasis in yeast.
Currently there is no direct assay to measure the activation of Arl1 GTPases.
The Arl1 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Arl1-GTP, but not Arl1-GDP. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.
Assay Principle
The Arl1 Activation Assay Kit uses configuration-specific anti-Arl1-GTP Mouse monoclonal antibody to measure Arl1-GTP levels in cell extracts or in vitro GTPγS loading Arl1 activation assays. Anti-Arl1-GTP mouse monoclonal antibody is first incubated with cell lysates containing Arl1-GTP. Next, the GTP-bound Arl1 is pulled down by protein A/G agarose. Finally, the precipitated Arl1-GTP is detected through immunoblot analysis using anti-Arl1 mouse monoclonal antibody.
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho
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