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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin
- 亚型:
lgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
FITC
- 适应物种:
脊椎动物
- 保质期:
24个月
- 抗原来源:
蛋白
- 目录编号:
TD-26072
- 级别:
科研级
- 库存:
999
- 供应商:
武汉天德
- 宿主:
Mouse
- 应用范围:
ELISA, WB, IHC
- 浓度:
1mg/ml
- 靶点:
R175H
- 抗体英文名:
Anti TP53(R175H) Mouse Monoclonal Antibody
- 抗体名:
TP53(R175H)
- 规格:
100 μL
TP53(R175H)
| Cat. #: TD-26072 |
| Gene Symbol: TP53(R175H) |
| Description: Anti-TP53(R175H) Mouse Monoclonal Antibody |
| Background: TP53 belongs to the p53 proteins family. It is ubiquitously expressed, acts as a tumor suppressor in many tumor types and induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Defects in TP53 are a cause of esophageal cancer (ESCR), lung cancer (LNCR), adrenocortical carcinoma (ADCC), choroid plexus papilloma (CPLPA) and so on. |
| Immunogen: A synthetic peptide from the internal region of TP53 which includes the mutation of R175H, human origin |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-TP53(R175H) antibody recognizes TP53(R175H) of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing. |
Western blot:
Immunofluorescence:
|
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文献和实验Science:直击肿瘤命门,Shibin Zhou 等开发靶向 TP53 突变的双特异性抗体
p53-targeted immunotherapyEurekAlert Science NewsR175H,其中 175 位的精氨酸被组氨酸取代,是 TP53 中观察到的最常见的突变,也是任何肿瘤抑制基因中最常见的突变。HMTEVVRHC (带下划线的为突变氨基酸) 来源于 p53R175H,与人类白细胞抗原 (HLA) 等位基因 (A*02:01) 结合,该等位基因存在于超过 40% 的美国高加索人中。近年来,TCR-Mimic (TCRm) 抗体已成为一类靶向肿瘤细胞内蛋白 pHLA(多肽 - MHC 复合
;(B)绿色荧光蛋白(GFP)标记的TYK-nu与T细胞共培养,E:T比为5:1,有或没有H2-scDb在Incucyte®系统中连续监测,显示24小时和96小时拍摄的相位和绿色荧光图像。 研究人员基于 CRISPR 的技术对携带内源性 HLA-A*02:01 和 p53R175H 的 KMS26、KLE 和 TYK-nu 癌细胞系中的 TP53 进行了基因破坏。H2-scDb 介导的细胞毒性通过破坏这些细胞中的 TP53 类似地减轻,通过Incucyte® 连续检测超过120h,呈现动态的定量
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
7.16 anti-rat IgG2b). -------------------------------------------------------------------------------- 5) For full details see: Clark, M.R., & Waldmann, H. Production of murine monoclonal antibodies in Methods in Hematology 13:1-20 (1986
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