- ￥4800
- Tanda
- 美国
- TD-26479
- 2025年10月29日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
- 亚型：
lgG
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
单克隆
- 标记物：
FITC
- 适应物种：
脊椎动物
- 保质期：
24个月
- 抗原来源：
蛋白
- 目录编号：
TD-26479
- 级别：
科研级
- 库存：
999
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
ELISA, WB, IHC
- 浓度：
1mg/ml
- 靶点：
A146V
- 抗体英文名：
Anti-Ras (A146V) mouse monoclonal antibody
- 抗体名：
RAS(A146V)
- 规格：
100 μL
RAS(A146V)
| Cat. #: TD-26479 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti-RAS(A146V) Mouse Monoclonal Antibody |
| Background: The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes A146V mutant, but not wild type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant RAS(A146V) and wild type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-RAS(A146V) monoclonal antibody (Cat. #TD-26479).
Immunofluorescence: 
Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146V) plasmid, pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146P) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRAS(A146V) monoclonal antibody (Cat. #TD-26479).
|
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人可溶性CD146(sCD146)酶联免疫分析（ELISA）
人 可溶性CD146(sCD146) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中可溶性CD146(sCD146) 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人可溶性 CD146(sCD146) 水平。用纯化的人可溶性 CD146(sCD146) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入
Q：Annexin V 凋亡检测试剂盒的原理是什么？ A：Annexin V 是一种钙离子依赖性磷脂结合蛋白，与磷脂酰丝氨酸 PS 有高度亲和力，可通过细胞外侧暴露的 PS 与凋亡早期细胞胞膜结合，将 Annexin V 标记荧光染料如 FITC 利用流式细胞仪或荧光显微镜可检测细胞凋亡。 碘化丙啶(Propidium Iodide, PI)或 7-AAD 可与双链 DNA 特异性结合，并产生强烈的荧光，正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性，核染料 PI 或 7-AAD
Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
技术资料暂无技术资料 索取技术资料
