- ￥4800
- Tanda
- TD-26479
- 美国
- 2025年11月04日
- ELISA, WB, IHC
- Mouse
- 脊椎动物
12 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 供应商：
武汉天德
- 库存：
999
- 靶点：
A146V
- 级别：
科研级
- 目录编号：
TD-26479
- 克隆性：
单克隆
- 抗原来源：
蛋白
- 保质期：
24个月
- 抗体英文名：
Anti-Ras (A146V) mouse monoclonal antibody
- 抗体名：
RAS(A146V)
- 标记物：
FITC
- 宿主：
Mouse
- 适应物种：
脊椎动物
- 免疫原：
A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin.
- 亚型：
IgG
- 形态：
液体
- 应用范围：
ELISA, WB, IHC
- 保存条件：
-20℃
- 浓度：
1mg/ml
- 规格：
100ul
RAS(A146V)
| Cat. #: TD-26479 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
| Description: Anti-RAS(A146V) Mouse Monoclonal Antibody |
| Background: The A146V mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146V mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146V, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes A146V mutant, but not wild type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant RAS(A146V) and wild type proteins. Purified His-tagged RAS(A146V) protein (lane 1) and corresponding wild type protein (lane 2) were blotted with Anti-RAS(A146V) monoclonal antibody (Cat. #TD-26479).
Immunofluorescence: 
Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146V) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146V) plasmid, pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146P) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRAS(A146V) monoclonal antibody (Cat. #TD-26479).
|
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
RAS(A146V)
￥4800
